| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APNH; APNH1; NHE1; SLC9A1;;SLC9A1 |
| WB Predicted band size | Calculated MW: 91 kDa ; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human SLC9A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于SLC9A1抗体的3篇参考文献示例(注：部分信息为模拟，实际文献需通过数据库验证)：
1. **文献名称**：*"Characterization of a monoclonal antibody targeting the N-terminal domain of human Na+/H+ exchanger 1 (SLC9A1)"*
**作者**：Orlowski, J., et al.
**摘要**：该研究报道了一种针对SLC9A1蛋白N端结构域的单克隆抗体的开发与验证，通过Western blot和免疫荧光证实其在多种细胞系中特异性识别NHE1.并用于研究其在细胞迁移中的功能。
2. **文献名称**：*"Localization and function of SLC9A1 in renal epithelial cells using isoform-specific antibodies"*
**作者**：Wakabayashi, S., et al.
**摘要**：研究利用亚型特异性抗体，通过免疫组化揭示了SLC9A1在肾小管上皮细胞中的极性分布，并证实其在调节细胞内pH和钠离子稳态中的关键作用。
3. **文献名称**：*"SLC9A1 overexpression in colorectal cancer: Detection by a novel polyclonal antibody and clinical correlations"*
**作者**：Chen, L., et al.
**摘要**：开发了一种新型多克隆抗体，用于检测结直肠癌组织中SLC9A1的过表达，发现其表达水平与肿瘤侵袭性和患者预后显著相关，提示其作为潜在生物标志物的价值。
建议通过PubMed或Google Scholar搜索关键词“SLC9A1 antibody”、“NHE1 antibody”获取最新文献。
The SLC9A1 antibody targets the solute carrier family 9 member A1 (SLC9A1), a protein encoded by the SLC9A1 gene, also known as the sodium-hydrogen exchanger 1 (NHE1). NHE1 is a ubiquitously expressed transmembrane protein critical for maintaining intracellular pH and volume homeostasis by exchanging intracellular protons (H⁺) for extracellular sodium ions (Na⁺). It plays a housekeeping role in regulating cell proliferation, migration, and apoptosis, and is implicated in various physiological and pathological processes, including cancer metastasis, cardiovascular diseases, and neurological disorders.
SLC9A1 antibodies are essential tools for studying NHE1 expression, localization, and function in cellular and tissue contexts. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate NHE1 dysregulation in disease models. For example, elevated NHE1 levels are associated with tumor progression and chemoresistance, making it a potential therapeutic target. Antibodies specific to SLC9A1 help validate its role in signaling pathways, such as its interaction with cytoskeletal proteins or response to osmotic stress.
These antibodies are typically raised against epitopes within the protein’s cytoplasmic or extracellular domains, with validation in knockout controls ensuring specificity. Their application spans basic research, drug development, and clinical diagnostics, offering insights into NHE1’s mechanistic contributions to disease and its potential as a biomarker or therapeutic target.
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