| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CALM; CLTH; LAP; PICALM;;PICALM |
| WB Predicted band size | Calculated MW: 71 kDa ; Observed MW: 71,69,66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human PICALM |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇涉及PICALM抗体的研究文献摘要概述(虚构示例，仅供格式参考)：
1. **文献名称**: "PICALM modulates autophagy and amyloid-β clearance in Alzheimer's disease models"
**作者**: Smith A, et al.
**摘要**: 研究利用PICALM特异性抗体，发现PICALM通过调控自噬体形成促进β-淀粉样蛋白(Aβ)的清除，抗体阻断实验证实其在阿尔茨海默病模型中的神经保护作用。
2. **文献名称**: "Interaction of PICALM with the clathrin-mediated endocytic machinery"
**作者**: Jones B, et al.
**摘要**: 通过免疫共沉淀(使用PICALM抗体)和细胞成像技术，揭示PICALM在网格蛋白包被囊泡形成中的关键作用，及其与AP2复合物的功能关联。
3. **文献名称**: "PICALM antibody-based biomarker analysis in cerebrospinal fluid of dementia patients"
**作者**: Lee C, et al.
**摘要**: 开发高灵敏度PICALM抗体ELISA检测法，发现脑脊液中PICALM水平与tau蛋白病理呈负相关，提示其作为阿尔茨海默病生物标志物的潜力。
(注：以上文献为示例性质，实际研究中需查询具体数据库获取真实文献。)
PICALM (Phosphatidylinositol-Binding Clathrin Assembly Protein) is a ubiquitously expressed adaptor protein involved in clathrin-mediated endocytosis, membrane trafficking, and autophagy. It plays a critical role in synaptic vesicle recycling, receptor internalization, and cargo sorting. The PICALM gene has been linked to several diseases, notably Alzheimer’s disease (AD), where genome-wide studies identified PICALM as a susceptibility locus. PICALM modulates amyloid precursor protein (APP) processing, influencing Aβ production and clearance, making it a focus in AD research. Additionally, PICALM is implicated in cancer, particularly acute leukemia, due to chromosomal translocations forming oncogenic fusion genes (e.g., PICALM-MLLT10).
PICALM antibodies are essential tools for studying its expression, localization, and interactions. They are used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate PICALM’s role in cellular pathways and disease mechanisms. Commercial antibodies target specific epitopes, enabling isoform detection or post-translational modification analysis. Validation in knockout models ensures specificity. Research utilizing these antibodies has advanced understanding of PICALM’s dual roles in neurobiology and oncology, highlighting its therapeutic potential. However, antibody variability requires careful optimization to avoid off-target effects, emphasizing the need for rigorous validation in experimental systems.
×
