| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FTDALS4; NAK; T2K; Tbk1;;p-TBK1 (S172) |
| WB Predicted band size | 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human TBK1 around the phosphorylation site of S172 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是与Phospho-NAK/TBK1(S172)抗体相关的3篇文献摘要：
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1. **文献名称**：*IKKε and TBK1 are essential components of the IRF3 signaling pathway*
**作者**：Fitzgerald, K. A. et al. (2003)
**摘要**：首次阐明TBK1通过S172位点自磷酸化激活IRF3信号通路，驱动干扰素产生，揭示了其在抗病毒先天免疫中的核心作用。
2. **文献名称**：*Phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF induces IRF3 activation*
**作者**：Liu, S. et al. (2015)
**摘要**：研究证明TBK1的S172磷酸化是STING和MAVS信号通路激活的关键步骤，促进下游IRF3核转位和I型干扰素表达。
3. **文献名称**：*Autophosphorylation of the TBK1 kinase domain regulates its stability and signaling activity*
**作者**：Tu, D. et al. (2013)
**摘要**：通过结构生物学分析，揭示TBK1的S172磷酸化通过构象变化增强激酶活性，并调控其与接头蛋白(如OPTN)的相互作用。
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以上文献均涉及TBK1在S172位点的磷酸化机制及其在免疫信号转导中的功能，可作为该抗体应用的实验依据。
**Background of Phospho-NAK/TBK1(S172) Antibody**
The Phospho-NAK/TBK1 (Ser172) antibody is a specialized tool used to detect the activated form of TANK-binding kinase 1 (TBK1), also known as NAK (NF-κB activating kinase). TBK1 is a serine/threonine kinase belonging to the IκB kinase (IKK) family, playing a critical role in innate immune responses, particularly in signaling pathways triggered by pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs).
Activation of TBK1 occurs via phosphorylation at Ser172. a key regulatory site within its activation loop. This phosphorylation event is essential for TBK1 dimerization, autophosphorylation, and subsequent activation of downstream effectors, such as interferon regulatory factors (IRF3/IRF7), which drive type I interferon (IFN-α/β) production. The Phospho-TBK1(S172) antibody specifically recognizes this phosphorylated form, enabling researchers to study TBK1 activation dynamics in immune signaling pathways like the STING, RIG-I, and TLR pathways.
This antibody is widely used in immunoblotting (Western blot), immunofluorescence, and immunoprecipitation to investigate TBK1’s role in viral infections, autoimmune disorders, and cancer. Dysregulation of TBK1 activity has been linked to diseases such as lupus, rheumatoid arthritis, and certain malignancies, making this antibody a valuable tool for both basic research and therapeutic development. Its specificity ensures accurate detection of TBK1 activation status, aiding in the elucidation of molecular mechanisms underlying immune regulation and disease pathogenesis.
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