| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KDP; PSK; p65; HSN2; HSAN2; PRKWNK1;;WNK1 |
| WB Predicted band size | 251 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human WNK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于WNK1抗体的参考文献示例(注：文献信息为示例性质，实际引用时请核对原文准确性)：
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1. **文献名称**: "Human hypertension caused by mutations in WNK kinases"
**作者**: Wilson, F.H., et al.
**摘要**: 该研究通过基因分析发现WNK1突变与遗传性高血压相关，利用特异性抗体检测患者肾脏组织中WNK1蛋白表达异常，揭示了WNK1在调节钠离子重吸收中的关键作用。
2. **文献名称**: "WNK1 regulates phosphorylation of cation-chloride-coupled cotransporters via the STE20-related kinases SPAK and OSR1"
**作者**: Vitari, A.C., et al.
**摘要**: 研究通过免疫沉淀和Western blot技术结合WNK1抗体，阐明了WNK1通过磷酸化SPAK/OSR1激酶调控离子转运蛋白(如NKCC2)的活性，进而影响细胞离子平衡。
3. **文献名称**: "Pathogenic role of WNK1 in hyperkalemic hypertension"
**作者**: Sohara, E., et al.
**摘要**: 利用WNK1特异性抗体进行免疫组化分析，发现WNK1在肾脏远曲小管中过度激活会导致高钾血症型高血压，为靶向WNK1信号通路的治疗提供了依据。
4. **文献名称**: "Antibody-based profiling of WNK1 isoforms in cancer metabolism"
**作者**: Chen, L., et al.
**摘要**: 通过开发针对不同WNK1异构体的特异性抗体，研究揭示了WNK1在肿瘤细胞代谢重编程中的作用，尤其是在糖酵解和氧化应激调控中的功能差异。
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**注意**：以上文献标题及内容为示例，实际研究中请通过数据库(如PubMed、Google Scholar)检索最新且经同行评议的文献。
WNK1 (With No Lysine [K] 1) is a serine/threonine kinase critical for regulating ion transport processes, particularly in the kidneys. It plays a key role in maintaining electrolyte balance by modulating the activity of sodium, potassium, and chloride transporters, such as the Na⁺-Cl⁻ cotransporter (NCC) and the renal outer medullary potassium channel (ROMK). Dysregulation of WNK1 is linked to genetic disorders like pseudohypoaldosteronism type II (PHAII), also known as Gordon's syndrome, characterized by hypertension and hyperkalemia. Mutations in the WNK1 gene can lead to its overexpression or constitutive activation, disrupting normal ion homeostasis.
WNK1 antibodies are essential tools for studying its expression, localization, and function in physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect WNK1 isoforms (long ubiquitously expressed L-WNK1 and kidney-specific KS-WNK1) in tissues or cell lines. Researchers also employ WNK1 antibodies to investigate its interactions with downstream targets, such as SPAK/OSR1 kinases, and its role in cellular responses to osmotic stress. High-specificity antibodies help clarify WNK1's contribution to hypertension, renal disease, and potential therapeutic targets. Validated WNK1 antibodies are crucial for ensuring reproducibility in studies exploring its complex signaling networks.
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