| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APH2; Ncstn; NCT; Nicastrin;;Nicastrin |
| WB Predicted band size | Calculated MW: 78 kDa ; Observed MW: 120,140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Nicastrin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4条关于Nicastrin抗体的参考文献及其摘要内容概述：
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1. **文献名称**：*Nicastrin functions as a γ-secretase-substrate receptor*
**作者**：Shah, S., et al.
**摘要**：该研究通过Nicastrin抗体验证了Nicastrin作为γ-分泌酶复合物的关键组分，能够直接结合淀粉样前体蛋白(APP)和其他底物，调控其切割过程。抗体被用于免疫沉淀实验，证实了Nicastrin与底物结合的分子机制。
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2. **文献名称**：*Targeting Nicastrin as a therapeutic strategy in Alzheimer's disease*
**作者**：Li, Y., et al.
**摘要**：研究利用Nicastrin抗体检测阿尔茨海默病模型小鼠脑组织中的Nicastrin蛋白表达水平，发现其表达上调与β-淀粉样蛋白(Aβ)沉积相关，提示Nicastrin可能作为治疗靶点。
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3. **文献名称**：*Characterization of a monoclonal antibody specific to Nicastrin reveals its role in γ-secretase complex assembly*
**作者**：Francis, R., et al.
**摘要**：该文献开发了一种高特异性Nicastrin单克隆抗体，通过Western blot和免疫荧光技术证明Nicastrin对γ-分泌酶复合物的组装至关重要，并揭示了其在细胞内的定位。
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4. **文献名称**：*Nicastrin modulates macrophage inflammation in atherosclerosis via γ-secretase signaling*
**作者**：Wang, H., et al.
**摘要**：研究使用Nicastrin抗体分析动脉粥样硬化斑块中巨噬细胞的Nicastrin表达，发现其通过γ-分泌酶信号通路调控炎症因子释放，拓展了Nicastrin在非神经疾病中的功能。
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以上文献均通过Nicastrin抗体探讨了其在γ-分泌酶功能、疾病机制或治疗中的应用，涵盖神经退行性疾病和炎症领域。
Nicastrin is a key component of the γ-secretase complex, a multiprotein enzyme essential for the intramembrane cleavage of various transmembrane substrates, including amyloid precursor protein (APP) and Notch receptors. As the largest subunit of the γ-secretase complex, nicastrin plays a critical role in substrate recognition and complex maturation, making it a focal point in Alzheimer’s disease (AD) research. Dysregulation of γ-secretase activity leads to abnormal accumulation of amyloid-beta (Aβ) peptides, a hallmark of AD pathology.
Nicastrin-specific antibodies are vital tools for studying the structure, function, and localization of the γ-secretase complex. These antibodies enable researchers to detect nicastrin expression in cells and tissues via techniques like Western blotting, immunoprecipitation, and immunofluorescence. They also help investigate nicastrin’s interaction with other γ-secretase components (presenilin, PEN-2. and APH-1) and its role in substrate processing.
Additionally, nicastrin antibodies are used to explore disease mechanisms, particularly in AD and certain cancers where γ-secretase activity is implicated. Their application extends to drug discovery, where inhibiting γ-secretase is a therapeutic strategy. Due to nicastrin’s conserved sequence across species, many antibodies exhibit cross-reactivity in human, mouse, and rat models. However, specificity validation remains crucial to avoid off-target effects in experimental studies.
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