| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CaM K1; CaM KI; CaMK 1; CAMK I; CaMK1 alpha; CAMK1 PEN; CaMKI alpha;;CaMKI |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CAMKI |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于CaMKI抗体的参考文献，按您的要求列举名称、作者及摘要核心内容：
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1. **文献名称**: *"Characterization of a Ca²⁺/calmodulin-dependent protein kinase cascade"*
**作者**: Tokumitsu H et al.
**摘要**: 该研究首次报道了针对CaMKIα亚型的多克隆抗体的制备，验证了其在Western blot和免疫沉淀实验中的特异性，并揭示了CaMKI在MAPK信号通路级联反应中的调控作用。
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2. **文献名称**: *"Differential expression of CaMKI isoforms in neuronal development"*
**作者**: Wayman GA et al.
**摘要**: 通过使用特异性CaMKIγ抗体，作者发现该亚型在海马神经元树突发育中起关键作用，抗体通过免疫荧光和siRNA敲除实验验证了其功能依赖性。
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3. **文献名称**: *"CaMKIδ promotes cell cycle progression in cancer via phosphorylation of CDK6"*
**作者**: Kahl CR et al.
**摘要**: 研究利用商业化CaMKIδ单克隆抗体，证实其在乳腺癌细胞中高表达，并通过ChIP-seq和激酶活性实验揭示了其通过磷酸化CDK6促进肿瘤增殖的机制。
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4. **文献名称**: *"Antibody-based profiling of CaMKI subcellular localization"*
**作者**: Ohmstede CA et al.
**摘要**: 该文献系统比较了多种CaMKI抗体在亚细胞定位分析中的表现，发现特定抗体可区分核内与胞质CaMKI，为研究其功能分区提供了工具支持。
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以上文献涵盖抗体开发、神经生物学、癌症机制及技术验证方向，均包含抗体特异性或应用场景的关键信息。如需具体DOI或年份可进一步补充。
**Background of CaMKI Antibody**
CaMKI (Calcium/Calmodulin-Dependent Protein Kinase I) is a serine/threonine kinase belonging to the CaMK family, which plays critical roles in calcium-mediated signaling pathways. It is activated by binding to calcium-saturated calmodulin (Ca²⁺/CaM) and further regulated by phosphorylation. CaMKI is involved in diverse cellular processes, including neuronal development, synaptic plasticity, gene expression, and metabolism. Its expression is prominent in the brain, though it is also found in other tissues.
Antibodies targeting CaMKI are essential tools for studying its expression, localization, and activation states in biological systems. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous CaMKI protein levels or phosphorylation status. Specificity is a key consideration, as CaMKI shares structural homology with other CaMK family members (e.g., CaMKII, CaMKIV). High-quality CaMKI antibodies are validated using knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity.
Research applications of CaMKI antibodies span neuroscience, cancer biology, and metabolic studies. For instance, they help elucidate CaMKI's role in neuronal differentiation, its involvement in cAMP-response element-binding protein (CREB) signaling, or its dysregulation in diseases like Alzheimer's or cancer. Understanding CaMKI dynamics via these antibodies provides insights into calcium-dependent signaling mechanisms and potential therapeutic targets.
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