| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hat1; KAT1;;KAT1 |
| WB Predicted band size | Calculated MW: 50 kDa ; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human KAT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于KAT1/HAT1抗体的3篇参考文献示例(注：部分为虚构示例，实际文献需通过数据库验证)：
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1. **文献名称**: *"HAT1-mediated acetylation orchestrates chromatin assembly and genomic stability"*
**作者**: Miller, C.L. et al.
**摘要**: 本研究利用HAT1特异性抗体，通过染色质免疫沉淀(ChIP)和免疫印迹技术，揭示了HAT1在组蛋白H4乙酰化及DNA损伤修复中的关键作用，证明其缺失导致染色质结构异常和基因组不稳定性。
2. **文献名称**: *"Development and validation of a monoclonal antibody targeting human KAT1/HAT1 for epigenetic studies"*
**作者**: Zhang, Y. et al.
**摘要**: 文章报道了一种高特异性抗KAT1/HAT1单克隆抗体的开发，通过免疫荧光和敲除细胞系验证其特异性，并应用于多种癌症模型中HAT1蛋白表达的定量分析。
3. **文献名称**: *"HAT1 regulates pluripotency in embryonic stem cells through histone acetylation"*
**作者**: Tanaka, R. et al.
**摘要**: 研究使用HAT1抗体进行免疫共沉淀(Co-IP)和染色质分析，发现HAT1通过调控多能性相关基因(如OCT4)的组蛋白乙酰化状态，影响胚胎干细胞的自我更新和分化。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词(如“HAT1 antibody application”或“KAT1 antibody characterization”)获取最新研究。
KAT1/HAT1 (Histone Acetyltransferase 1) is a conserved enzyme involved in chromatin modification and epigenetic regulation. As a member of the type B histone acetyltransferase family, HAT1 catalyzes the acetylation of lysine residues on newly synthesized histone H4. particularly at lysine 12 (H4K12), which is critical for chromatin assembly during DNA replication and repair. This enzyme operates in the cytoplasm, where it acetylates free histones prior to their incorporation into nucleosomes, ensuring proper chromatin structure and gene expression regulation. HAT1 also interacts with histone chaperones like Hat2 and Hif1 to form complexes that stabilize histones and facilitate their nuclear transport.
Antibodies targeting HAT1/KAT1 are essential tools for studying its expression, localization, and function in diverse biological processes, including DNA replication, cell cycle progression, and transcriptional regulation. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect HAT1 in human, mouse, or rat samples. Researchers employ HAT1-specific antibodies to explore its role in diseases, particularly cancer, where aberrant histone acetylation is linked to oncogenesis and metastasis. Additionally, these antibodies help investigate HAT1's non-canonical roles, such as its involvement in mitochondrial metabolism and immune responses. Validation of HAT1 antibodies typically includes testing for specificity via knockout cell lines or siRNA knockdown to ensure accurate detection, given the structural similarities among acetyltransferase family members.
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