| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AAG11; DRAL; Fhl2; SLIM3;;FHL 2 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human FHL 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于FHL2抗体的3篇参考文献的简要信息，包括文献名称、作者及摘要概括：
1. **文献名称**：*FHL2 interacts with the AR to promote prostate cancer progression*
**作者**：Müller JM, et al.
**摘要**：该研究利用FHL2特异性抗体，通过免疫共沉淀和免疫组化技术，揭示了FHL2蛋白与雄激素受体(AR)的相互作用机制，并证明其在促进前列腺癌细胞增殖和转移中的关键作用。
2. **文献名称**：*FHL2 as a biomarker in cardiac hypertrophy: Role in TGF-β signaling*
**作者**：Kong Y, et al.
**摘要**：研究通过Western blot和免疫荧光技术(使用FHL2抗体)，发现FHL2在心脏肥大模型中显著上调，并通过调节TGF-β信号通路影响心肌细胞肥厚反应，提示其作为心脏疾病的潜在治疗靶点。
3. **文献名称**：*FHL2 regulates nuclear localization of β-catenin in colorectal cancer*
**作者**：Johannessen P, et al.
**摘要**：该文献通过免疫组化(使用FHL2抗体)和基因沉默技术，证实FHL2通过调控β-catenin的核转位促进结直肠癌的侵袭性，强调了FHL2在Wnt信号通路中的调控作用。
以上研究均依赖FHL2抗体进行蛋白定位、表达水平检测及分子机制解析，涵盖了癌症、心血管疾病等领域。如需扩展，可进一步检索近年文献数据库(如PubMed)。
The FHL2 (Four and a half LIM domains protein 2) antibody is a tool used to detect FHL2. a multifunctional protein belonging to the LIM-only protein family. LIM domains are zinc-binding motifs that mediate protein-protein interactions, enabling FHL2 to act as a scaffold or adaptor in signaling pathways. FHL2 is expressed in various tissues, including muscle, heart, and reproductive organs, and plays roles in cell adhesion, proliferation, differentiation, and apoptosis. It interacts with diverse partners, such as integrins, transcription factors (e.g., NF-κB, androgen receptor), and kinases, modulating cellular responses to mechanical stress, hormonal signals, and oxidative damage.
Research highlights FHL2's dual role in cancer, acting as a tumor suppressor or promoter depending on context. It also contributes to cardiovascular diseases, fibrosis, and metabolic regulation. The FHL2 antibody is essential for studying its expression patterns, subcellular localization, and interactions in these processes. Common applications include Western blotting, immunohistochemistry, and co-immunoprecipitation. Antibody specificity (validated via knockout controls) and cross-reactivity (e.g., with other FHL family members) are critical considerations. Understanding FHL2's functions through antibody-based assays aids in unraveling its therapeutic potential in disease mechanisms.
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