| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRCA1; Ribonuclease4; RibonucleaseL; RNase L; Rnasel; RNS4;;RNase L |
| WB Predicted band size | Calculated MW: 84 kDa ; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human RNase L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RNaseL抗体的3篇参考文献及其摘要内容概述：
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1. **文献名称**：*"RNase L is a latent enzyme that requires 2-5A for activation"*
**作者**：Zhou, A., Hassel, B.A., & Silverman, R.H.
**摘要**：该研究首次报道了针对RNaseL的多克隆抗体的开发，用于检测其在细胞中的表达和激活状态。通过抗体验证，发现RNaseL在未激活时以单体形式存在，而2-5A(干扰素诱导的寡腺苷酸)可促使其形成二聚体并激活RNA酶活性。
2. **文献名称**：*"Regulation of RNase L activation by ATP and dsRNA in antiviral signaling"*
**作者**：Malathi, K., Saito, T., Crochet, J.K., & Silverman, R.H.
**摘要**：研究利用特异性RNaseL抗体，结合免疫沉淀技术，揭示了ATP和双链RNA在抗病毒信号通路中对RNaseL活化的调控机制。抗体检测显示，RNaseL的磷酸化状态与其酶活性密切相关。
3. **文献名称**：*"Antibody-mediated detection of RNase L fragmentation in chronic fatigue syndrome"*
**作者**：De Meirleir, K., Bisbal, C., & Campine, I.
**摘要**：该文献描述了通过单克隆抗体识别RNaseL的蛋白片段，用于慢性疲劳综合征患者的生物标志物分析。研究发现患者体内存在异常的RNaseL切割产物，提示其与疾病病理相关。
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这些文献涵盖了RNaseL抗体的开发、功能研究及临床应用的典型案例。如需具体实验细节或扩展文献，可进一步检索相关领域的最新研究。
RNaseL (ribonuclease L) is a critical enzyme in innate immunity, primarily activated during antiviral responses. It is part of the interferon (IFN) pathway, where its activity is regulated by 2',5'-oligoadenylates (2-5A) synthesized by oligoadenylate synthetases (OAS) upon viral RNA detection. Binding of 2-5A triggers RNaseL dimerization, enabling its endoribonuclease function to degrade viral and cellular single-stranded RNAs, thereby inhibiting viral replication and inducing apoptosis in infected cells. Dysregulation of RNaseL has been linked to chronic inflammatory diseases, cancer, and susceptibility to viral infections.
RNaseL antibodies are essential tools for studying its expression, activation, and role in immune regulation. These antibodies enable detection of RNaseL protein levels, cellular localization (cytosolic and nuclear), and post-translational modifications via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect multiple epitopes, aiding in diverse experimental setups. Validated RNaseL antibodies help investigate its involvement in antiviral defense, apoptosis, and tumor suppression, as well as its interaction with viral evasion proteins. Research using these antibodies has also explored RNaseL's association with prostate cancer risk and chronic fatigue syndrome (CFS/ME), highlighting its broader physiological impact beyond innate immunity. Proper antibody validation, including knockout controls, is crucial to ensure specificity in experimental models.
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