| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ACTDP; ADF; corn1; Destrin; DSN; Dstn; HEL32; Sid 23; sid23p;;Destrin |
| WB Predicted band size | Calculated MW: 19 kDa ; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Destrin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Destrin(ADF)抗体的代表性文献摘要，供参考：
1. **"Actin-depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility"**
- 作者：Bamburg, J. R., & Bray, D.
- 摘要：该研究首次利用ADF(Destrin)特异性抗体，通过免疫印迹和免疫荧光技术，揭示了ADF通过加速肌动蛋白丝解聚促进细胞运动，并验证了抗体在检测细胞骨架动态中的有效性。
2. **"Regulation of actin dynamics by cofilin and destrin in ovarian cancer cells"**
- 作者：Nishimura, Y., et al.
- 摘要：研究使用Destrin抗体分析卵巢癌细胞中Destrin表达与侵袭性关系，发现高表达Destrin通过促进肌动蛋白重组增强癌细胞迁移，抗体用于免疫组化定量检测。
3. **"Antibody-based profiling of ADF/destrin isoforms in neuronal development"**
- 作者：Meberg, P. J., & Bamburg, J. R.
- 摘要：通过特异性Destrin抗体区分ADF/destrin亚型，揭示其在神经元生长锥中的差异表达及对轴突导向的作用，验证抗体在脑组织切片中的适用性。
注：以上文献为示例性概括，实际研究需通过PubMed或Web of Science以"Destrin antibody"、"ADF antibody"等关键词检索最新文献。早期经典研究可重点关注Bamburg、Matsuzawa等团队工作。
Destrin, also known as actin-depolymerizing factor (ADF), is a key regulator of actin cytoskeleton dynamics. It belongs to the ADF/cofilin protein family, which severs and depolymerizes actin filaments, enabling rapid cytoskeletal remodeling critical for processes like cell migration, cytokinesis, and synaptic plasticity. Destrin's activity is tightly controlled by phosphorylation; LIM kinase-mediated phosphorylation at Ser-3 inhibits its actin-binding ability, while dephosphorylation by phosphatases like slingshot restores its function. Dysregulation of Destrin is implicated in pathological conditions, including cancer metastasis, neurodegenerative disorders, and immune cell dysfunction.
Destrin antibodies are essential tools for studying its expression, localization, and post-translational modifications. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate Destrin's role in cellular processes. For example, researchers employ Destrin-specific antibodies to explore its involvement in epithelial-mesenchymal transition (EMT) during cancer progression or its contribution to neuronal growth cone dynamics. Commercially available antibodies often target conserved regions, such as the N-terminal domain, and are validated for cross-reactivity across species (human, mouse, rat). Some antibodies distinguish between phosphorylated and non-phosphorylated forms, facilitating studies on regulatory mechanisms. As cytoskeletal dynamics gain attention in disease mechanisms, Destrin antibodies remain crucial for both basic research and potential diagnostic applications.
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