| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC2; CRCS10; MDPL; POLD; POLD1;;POLD1 |
| WB Predicted band size | 124 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human POLD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于POLD1抗体的3篇参考文献示例(注：以下文献信息为示例性概括，具体内容需通过学术数据库验证)：
1. **文献名称**：*"POLD1 mutations drive immunotherapy resistance in human cancer by impairing DNA mismatch repair"*
**作者**：Wang, Y. et al.
**摘要**：研究揭示了POLD1突变通过破坏DNA错配修复(MMR)导致癌症免疫治疗耐药性，利用POLD1抗体进行Western blot和免疫组化分析，证实突变体蛋白表达异常与临床预后相关。
2. **文献名称**：*"Functional characterization of DNA polymerase delta catalytic subunit in genome maintenance"*
**作者**：Smith, J. & Jones, R.
**摘要**：通过免疫沉淀(IP)和免疫荧光技术，结合POLD1抗体，证明POLD1在复制应激反应中的核心作用，并发现其与BRCA1/2的协同修复机制。
3. **文献名称**：*"Antibody-based detection of POLD1 expression in colorectal cancer progression"*
**作者**：Li, H. et al.
**摘要**：利用特异性POLD1抗体分析结直肠癌组织样本，发现POLD1蛋白表达水平与肿瘤分期正相关，提示其作为潜在生物标志物的价值。
4. **文献名称**：*"Development and validation of a novel POLD1 monoclonal antibody for diagnostic applications"*
**作者**：Garcia, M. et al.
**摘要**：报道了一种高特异性POLD1单克隆抗体的开发，验证其在ELISA和免疫细胞化学中的灵敏度，为遗传性癌症综合征的诊断提供工具。
**建议**：具体文献可通过PubMed或Google Scholar以关键词“POLD1 antibody”“POLD1 function”“POLD1 cancer”检索获取。
The POLD1 antibody is a crucial tool for studying the DNA polymerase delta catalytic subunit, encoded by the *POLD1* gene. This enzyme plays a vital role in DNA replication and repair, specifically in synthesizing the lagging strand during replication and participating in base excision repair and homologous recombination repair. POLD1 is essential for maintaining genomic stability, and its dysfunction has been linked to cancer, aging, and genetic disorders like Mandibular Hypoplasia, Deafness, Progeroid Syndrome (MDPL).
Antibodies targeting POLD1 enable researchers to detect its expression, localization, and post-translational modifications in cells and tissues. They are widely used in techniques such as Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC). Studies employing these antibodies have revealed POLD1's overexpression in certain cancers (e.g., colorectal, endometrial) and its role in tumor progression. Additionally, POLD1 mutations (e.g., germline variants) are associated with increased cancer susceptibility and replication stress, making the antibody valuable for functional studies.
Commercial POLD1 antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown. However, variability in performance across applications necessitates careful optimization. Research using these antibodies continues to advance our understanding of DNA replication fidelity, DNA damage response, and therapeutic targeting of polymerase deficiencies.
×
