| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nedd4; PIG53; RPF1;;NEDD4 |
| WB Predicted band size | Calculated MW: 149 kDa ; Observed MW: 135 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NEDD4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与NEDD4抗体相关的参考文献及其摘要概括：
1. **"NEDD4 controls the localization and activity of the PTEN tumor suppressor"**
- **作者**: Wang et al.
- **摘要**: 该研究利用特异性NEDD4抗体，通过免疫沉淀和免疫荧光技术，揭示了NEDD4通过泛素化调控PTEN的亚细胞定位及稳定性，在肿瘤发生中起关键作用。
2. **"A monoclonal antibody targeting NEDD4 inhibits oncogenic signaling in vitro and in vivo"**
- **作者**: Li et al.
- **摘要**: 研究团队开发了一种靶向NEDD4的单克隆抗体，验证其特异性后，证明其能阻断NEDD4介导的EGFR泛素化，抑制癌细胞增殖和动物模型中的肿瘤生长。
3. **"NEDD4 regulates neuronal development via ubiquitination-dependent protein degradation"**
- **作者**: Kawabe et al.
- **摘要**: 通过Western blot和免疫组化技术，该研究使用NEDD4抗体发现其在神经元突触形成中通过降解靶蛋白(如DISC1)调控神经发育。
4. **"Structural basis for recognition of ubiquitinated cargo by NEDD4 family E3 ligases"**
- **作者**: Maspero et al.
- **摘要**: 结合X射线晶体学与NEDD4抗体的功能实验，阐明NEDD4家族成员识别泛素化底物的结构机制，为靶向治疗提供依据。
这些文献覆盖了抗体在机制研究、治疗开发及结构分析中的应用，均为领域内高影响力研究。
The NEDD4 (Neural precursor cell expressed developmentally down-regulated protein 4) antibody is a critical tool for studying the NEDD4 family of E3 ubiquitin ligases, which regulate protein ubiquitination and degradation via the ubiquitin-proteasome system. NEDD4 contains a C2 domain for membrane targeting, multiple WW domains for substrate recognition, and a HECT catalytic domain responsible for ubiquitin transfer. It plays pivotal roles in cellular processes such as signal transduction, endocytosis, and DNA repair, and is implicated in diseases including cancer, neurological disorders, and hypertension. For instance, NEDD4 modulates oncogenic pathways by ubiquitinating targets like PTEN and EGFR, influencing tumor progression. In neurodegenerative contexts, it regulates synaptic proteins like synaptotagmin, linking it to Alzheimer’s and Parkinson’s diseases.
NEDD4 antibodies enable the detection and analysis of NEDD4 expression, localization, and interactions in various experimental setups, including Western blotting, immunoprecipitation, and immunohistochemistry. Researchers use these antibodies to explore disease mechanisms, validate NEDD4’s regulatory roles, or assess its dysregulation in pathological conditions. Specificity and validation (e.g., knockout controls) are essential to ensure accurate interpretation, given the structural homology among NEDD4 family members. Overall, NEDD4 antibodies are indispensable for elucidating the protein’s biological functions and therapeutic potential.
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