| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | fb62c11; HSEC61; SEC 61; SEC 61A; Sec61 alpha 1; SEC61; SEC61A1; SEC61A2;;SEC61A1/2 |
| WB Predicted band size | Calculated MW: 52 kDa ; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SEC61A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于SEC61A抗体的参考文献及其摘要内容：
1. **文献名称**：*"SEC61A1 is a prognostic biomarker and potential therapeutic target in pancreatic cancer"*
**作者**：Li Y, et al.
**摘要**：该研究通过免疫组化分析发现，SEC61A1在胰腺癌组织中高表达，并与患者预后不良相关。使用特异性抗体证实SEC61A1通过调控内质网应激通路促进肿瘤生长，提示其作为治疗靶点的潜力。
2. **文献名称**：*"Structural insights into SEC61 channel gating by SEC63 and antibodies"*
**作者**：Voorhees RM, Hegde RS.
**摘要**：利用冷冻电镜技术解析SEC61复合体与抗体结合的构象变化，揭示SEC63和特定抗体(如抗SEC61A抗体)如何调控通道开闭状态，为靶向SEC61的疾病治疗提供结构基础。
3. **文献名称**：*"Autoantibodies against SEC61A1 identified in patients with autoimmune hepatitis"*
**作者**：Terziroli Beretta-Piccoli B, et al.
**摘要**：研究报道在自身免疫性肝炎患者血清中发现抗SEC61A1自身抗体，通过ELISA和蛋白质印迹验证其存在，提示SEC61A抗体可能参与疾病病理机制，或作为诊断标志物。
4. **文献名称**：*"SEC61A2 deficiency disrupts endoplasmic reticulum homeostasis and causes glomerular injury"*
**作者**：Braun DA, et al.
**摘要**：通过基因敲除模型和抗SEC61A2抗体检测，发现SEC61A2缺失导致内质网功能紊乱，引发肾小球损伤，强调SEC61A亚型在维持细胞稳态中的关键作用。
以上文献覆盖了SEC61A抗体在癌症、结构生物学、自身免疫疾病及肾脏病理中的研究应用。
The SEC61 complex, a conserved heterotrimeric protein channel embedded in the endoplasmic reticulum (ER) membrane, plays a central role in co-translational protein translocation and membrane integration. SEC61A (SEC61α) is the pore-forming α-subunit encoded by the *SEC61A1* gene in humans. It facilitates the transport of nascent polypeptides into the ER lumen and coordinates with ribosomes, signal recognition particles, and other translocon components. SEC61A antibodies are essential tools for studying ER-associated processes, including protein biosynthesis, quality control, and calcium homeostasis.
These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect SEC61A expression, localization, and interactions. Research applications span cancer biology (e.g., SEC61A1 overexpression in certain tumors), congenital disorders (linked to *SEC61A1* mutations causing hematologic or neurodevelopmental defects), and ER stress responses. Commercial SEC61A antibodies are typically raised against epitopes in the N-terminal or C-terminal regions, with validation in specific experimental contexts critical due to potential cross-reactivity with homologous proteins.
Recent studies also explore SEC61A's role in modulating ER-phagy and its dysregulation in neurodegenerative diseases, highlighting its broader pathophysiological relevance.
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