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Rabbit Monoclonal eIF4A3 Antibody

  • 中文名: eIF4A3抗体
  • 别    名: DDX48; EIF4A3; eIF4AIII; hNMP 265; NMP 265; NMP265; NUK34;;eIF4A3
货号: IPDX18168
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDDX48; EIF4A3; eIF4AIII; hNMP 265; NMP 265; NMP265; NUK34;;eIF4A3
WB Predicted band size47 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human eIF4A3
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3条关于eIF4A3抗体的参考文献(示例为虚构内容,实际文献需根据真实数据库检索):

1. **文献名称**: *"eIF4A3 is a critical component of the exon junction complex involved in nonsense-mediated mRNA decay"*

**作者**: Lejeune F., et al.

**摘要**: 本研究利用特异性eIF4A3抗体,通过免疫共沉淀和质谱分析,证实eIF4A3与外显子连接复合体(EJC)核心蛋白相互作用,并揭示其在无义介导的mRNA降解(NMD)中的调控作用。

2. **文献名称**: *"Antibody-based profiling of eIF4A3 expression in human cancers reveals its association with tumor progression"*

**作者**: Wang Y., et al.

**摘要**: 通过开发高特异性eIF4A3单克隆抗体,研究者分析了多种癌症组织中eIF4A3的蛋白表达水平,发现其过表达与肿瘤转移和不良预后显著相关,提示其作为潜在生物标志物的价值。

3. **文献名称**: *"Functional characterization of eIF4A3 in RNA splicing using CRISPR/Cas9 and antibody-mediated depletion"*

**作者**: Silva R., et al.

**摘要**: 结合CRISPR/Cas9基因编辑技术和eIF4A3抗体介导的蛋白敲降实验,证明eIF4A3在前体mRNA剪接中的必要性,并通过RNA-seq数据揭示其靶向调控的剪接事件。

如需真实文献,建议在PubMed或Web of Science检索关键词“eIF4A3 antibody”或“eIF4A3 function”,并筛选涉及抗体应用的论文。

背景信息

The eIF4A3 antibody is a crucial tool for studying the eukaryotic translation initiation factor 4A-III (eIF4A3), a DEAD-box RNA helicase involved in mRNA metabolism and quality control. As a core component of the exon junction complex (EJC), eIF4A3 binds to spliced mRNAs during nuclear export, marking exon-exon junctions and participating in nonsense-mediated mRNA decay (NMD), a surveillance mechanism that degrades transcripts with premature stop codons. Unlike other eIF4A family members (eIF4A1/2), eIF4A3 lacks canonical translation initiation functions but plays a specialized role in RNA processing and stability. Its ATP-dependent helicase activity facilitates EJC assembly and RNA remodeling. Researchers use eIF4A3 antibodies to investigate its localization, interactions, and expression in cellular pathways, particularly in studies of mRNA splicing, NMD, and stress granule dynamics. Dysregulation of eIF4A3 is linked to cancers and neurological disorders, making its antibody valuable for diagnostic or therapeutic research. These antibodies are commonly validated via Western blotting, immunofluorescence, or immunoprecipitation to ensure specificity, given the structural similarities among DEAD-box proteins. Understanding eIF4A3’s role enhances insights into post-transcriptional gene regulation and disease mechanisms.

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