Rabbit Monoclonal eIF4A3 Antibody

The eIF4A3 antibody is a crucial tool for studying the eukaryotic translation initiation factor 4A-III (eIF4A3), a DEAD-box RNA helicase involved in mRNA metabolism and quality control. As a core component of the exon junction complex (EJC), eIF4A3 binds to spliced mRNAs during nuclear export, marking exon-exon junctions and participating in nonsense-mediated mRNA decay (NMD), a surveillance mechanism that degrades transcripts with premature stop codons. Unlike other eIF4A family members (eIF4A1/2), eIF4A3 lacks canonical translation initiation functions but plays a specialized role in RNA processing and stability. Its ATP-dependent helicase activity facilitates EJC assembly and RNA remodeling. Researchers use eIF4A3 antibodies to investigate its localization, interactions, and expression in cellular pathways, particularly in studies of mRNA splicing, NMD, and stress granule dynamics. Dysregulation of eIF4A3 is linked to cancers and neurological disorders, making its antibody valuable for diagnostic or therapeutic research. These antibodies are commonly validated via Western blotting, immunofluorescence, or immunoprecipitation to ensure specificity, given the structural similarities among DEAD-box proteins. Understanding eIF4A3’s role enhances insights into post-transcriptional gene regulation and disease mechanisms.