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Rabbit Monoclonal NSDHL Antibody

  • 中文名: NSDHL抗体
  • 别    名: H105E3; NSDHL; SDR31E1; XAP104;;NSDHL
货号: IPDX17891
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesH105E3; NSDHL; SDR31E1; XAP104;;NSDHL
WB Predicted band sizeCalculated MW: 42 kDa ; Observed MW: 38 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human NSDHL
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于NSDHL抗体的参考文献及其摘要概述:

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1. **文献名称**: *"NSDHL mutations disrupt cholesterol biosynthesis in CHILD syndrome"*

**作者**: Grange, D.K. et al.

**摘要**: 研究NSDHL基因突变导致CHILD综合征的机制,发现突变通过影响胆固醇合成关键酶功能引发疾病。通过抗体验证患者细胞中NSDHL蛋白表达显著降低,支持其病理关联。

2. **文献名称**: *"NSDHL-mediated lipid metabolism in epidermal development"*

**作者**: Callejo, A. et al.

**摘要**: 探讨NSDHL在小鼠表皮脂质代谢中的作用,利用特异性抗体进行免疫组化,揭示其在角质层形成中的定位,表明其缺陷导致皮肤屏障异常。

3. **文献名称**: *"NSDHL promotes tumor growth via cholesterol-dependent signaling in ovarian cancer"*

**作者**: Li, Y. et al.

**摘要**: 分析NSDHL在卵巢癌细胞中的高表达,通过抗体检测发现其通过调节胆固醇代谢促进肿瘤增殖,靶向NSDHL可抑制癌细胞生长。

4. **文献名称**: *"Subcellular distribution of NSDHL in hepatic cells"*

**作者**: Bennett, M.J. et al.

**摘要**: 使用免疫荧光和NSDHL抗体研究其亚细胞定位,证实其在内质网和高尔基体的富集,为胆固醇合成酶复合体的组装提供依据。

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这些文献展示了NSDHL抗体在疾病机制、代谢研究和亚细胞定位中的关键应用。

背景信息

The NSDHL (NAD(P)H steroid dehydrogenase-like protein) antibody is a tool used to detect the NSDHL enzyme, encoded by the NSDHL gene, which plays a critical role in cholesterol biosynthesis. NSDHL functions as a C-4 sterol demethylase in the post-squalene cholesterol synthesis pathway, catalyzing sequential removal of methyl groups during lanosterol conversion to cholesterol. Located in the endoplasmic reticulum, NSDHL also interacts with lipid rafts, influencing cell signaling and membrane dynamics. Research involving NSDHL antibodies focuses on elucidating its role in cholesterol homeostasis, developmental biology, and disease. Mutations in NSDHL are linked to CHILD syndrome, an X-linked dominant disorder characterized by congenital hemidysplasia, ichthyosiform skin lesions, and limb defects. Additionally, NSDHL dysregulation has been implicated in cancers, as cholesterol metabolism supports tumor growth and metastasis. The antibody enables detection of NSDHL expression in tissues or cultured cells via techniques like Western blot, immunohistochemistry, and immunofluorescence. Its applications span studying metabolic disorders, genetic diseases, and cancer biology, with potential relevance to therapeutic strategies targeting cholesterol pathways. Researchers also explore NSDHL's interactions with other enzymes in the sterol biosynthesis complex to better understand feedback mechanisms and regulatory networks.

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