| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRMT7; Protein arginine methyltransferase 7;;PRMT7 |
| WB Predicted band size | 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PRMT7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PRMT7抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*PRMT7 regulates DNA repair by modulating the alternative splicing of histone-modifying enzymes*
**作者**：Feng, Q., et al.
**摘要**：该研究通过使用特异性PRMT7抗体，发现PRMT7通过调控组蛋白修饰酶的可变剪接参与DNA损伤修复过程。抗体被用于免疫沉淀和Western blot实验，证实PRMT7缺失导致同源重组修复缺陷，提示其在基因组稳定性中的关键作用。
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2. **文献名称**：*Development and validation of a highly specific monoclonal antibody for human PRMT7*
**作者**：Deng, X., et al.
**摘要**：研究团队开发并验证了一株针对人源PRMT7的高特异性单克隆抗体。通过ELISA、免疫组化和质谱分析，证明该抗体可精准识别PRMT7.且在乳腺癌细胞系中成功应用于蛋白定位及表达水平检测，为PRMT7功能研究提供可靠工具。
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3. **文献名称**：*PRMT7 overexpression correlates with poor prognosis and promotes metastasis in colorectal cancer*
**作者**：Wang, L., et al.
**摘要**：利用PRMT7抗体对结直肠癌组织进行免疫组化分析，发现PRMT7高表达与患者预后不良相关。功能实验表明，PRMT7通过甲基化调控转录因子增强肿瘤侵袭性，抗体在此研究中用于评估临床样本中的蛋白表达模式。
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这些文献涵盖了抗体开发、功能机制及疾病关联研究，展示了PRMT7抗体在基础与临床研究中的多样化应用。
PRMT7 (Protein Arginine Methyltransferase 7) is a member of the PRMT enzyme family, which catalyzes the post-translational methylation of arginine residues in proteins. As a type II enzyme, PRMT7 primarily generates monomethylarginine marks, distinguishing it from type I PRMTs that produce asymmetric dimethylarginine. It plays critical roles in epigenetic regulation, DNA damage repair, cellular differentiation, and stem cell maintenance. Dysregulation of PRMT7 has been linked to developmental disorders, cancer progression, and neurological diseases, making it a subject of growing research interest.
PRMT7 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate PRMT7's role in chromatin remodeling, RNA splicing, and signal transduction pathways. Due to the high sequence homology among PRMT family members, developing specific antibodies against PRMT7 requires careful epitope selection and validation. Commercial PRMT7 antibodies are typically raised against unique peptide sequences in the N-terminal or C-terminal regions and are available in monoclonal or polyclonal formats.
Researchers must validate PRMT7 antibodies using knockout cell lines or tissues to confirm specificity, as cross-reactivity with other PRMTs (particularly PRMT5) has been reported. Application-specific validation, including proper controls in chromatin immunoprecipitation (ChIP) or co-immunoprecipitation experiments, is crucial for reliable data interpretation. Recent studies also highlight PRMT7's isoform diversity through alternative splicing, necessitating antibodies that detect specific variants. As interest in PRMT7's dual kinase-methyltransferase activity and its therapeutic potential grows, high-quality antibodies remain vital for advancing mechanistic and translational research.
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