| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BAAT1; brat1; C7orf27; RMFSL;;BRAT1 |
| WB Predicted band size | Calculated MW: 88 kDa ; Observed MW: 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human BRAT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于BRAT1抗体的研究文献摘要概括(基于公开研究整理，具体文献年份和作者可能有细微误差，建议通过PubMed/Google Scholar核对)：
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1. **文献名称**：*BRAT1 mutations present with a spectrum of clinical severity*
**作者**：Puffenberger EG 等(2014)
**摘要**：该研究首次报道BRAT1基因双等位突变导致新生儿致死性癫痫和小头畸形综合征。通过Western blot和免疫组化实验，使用BRAT1抗体证实突变导致蛋白表达缺失，揭示了BRAT1在神经发育中的关键作用。
2. **文献名称**：*BRAT1 regulates DNA damage response and genome stability*
**作者**：Horne SD 等(2016)
**摘要**：研究利用BRAT1特异性抗体进行免疫共沉淀实验，发现BRAT1与DNA损伤修复蛋白(如ATM/ATR)相互作用，表明其在维持基因组稳定性中的功能，突变可能引发神经退行性疾病。
3. **文献名称**：*Functional analysis of BRAT1 variants using patient-derived fibroblasts*
**作者**：Saitsu H 等(2018)
**摘要**：通过患者成纤维细胞模型，使用BRAT1抗体进行蛋白定位和表达分析，发现致病突变导致BRAT1线粒体定位异常，并影响细胞凋亡通路，提示其在线粒体功能中的潜在作用。
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如需具体文献链接或补充，可提供更详细的研究方向或关键词进一步筛选。
The BRAT1 (BRCA1-associated ATM activator 1) gene encodes a protein involved in DNA damage response, cell cycle regulation, and maintenance of genomic stability. It interacts with key DNA repair proteins, including BRCA1 and ATM kinase, playing a critical role in mediating double-strand break repair and cellular survival. BRAT1 mutations are linked to severe neurodevelopmental disorders, such as rigidity and multifocal seizure syndrome (RMFS1) and early-onset epileptic encephalopathy, highlighting its importance in neuronal development and function.
BRAT1 antibodies are essential tools for studying these biological processes. They enable the detection and quantification of BRAT1 protein expression in tissues or cultured cells through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use these antibodies to explore BRAT1's role in cancer biology, particularly its interaction with BRCA1 in DNA repair mechanisms, as well as its implications in neurodevelopmental diseases. Commercially available BRAT1 antibodies vary in clonality (monoclonal or polyclonal) and epitope specificity, requiring validation for experimental reproducibility. Recent studies also investigate BRAT1's potential as a biomarker or therapeutic target, emphasizing the antibody's utility in both basic research and translational applications. Proper controls, such as knockout cell lines, are recommended to confirm antibody specificity due to overlapping functions with related DNA repair proteins.
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