| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NF A2; Oct 2; Oct-2; OTF-2; OTF2; POU2F2; ;45567 |
| WB Predicted band size | Calculated MW: 51 kDa ; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Oct 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于2-Oct(或Oct-2)抗体的虚构参考文献示例(仅供参考，非真实文献)：
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1. **文献名称**: *Oct-2 regulates B cell-specific immunoglobulin gene expression*
**作者**: Smith, J., et al.
**摘要**: 研究探讨了转录因子Oct-2在B淋巴细胞中对免疫球蛋白基因的调控作用，利用Oct-2抗体进行染色质免疫沉淀(ChIP)实验，证实其直接结合于IgH基因增强子区域。
2. **文献名称**: *Role of Oct-2 in autoimmune disorders: Insights from antibody-mediated knockdown models*
**作者**: Lee, H., & Martinez, R.
**摘要**: 通过Oct-2抗体阻断实验，发现Oct-2在多发性硬化症患者B细胞中异常高表达，提示其可能通过调控炎症因子参与自身免疫反应。
3. **文献名称**: *Structural characterization of Oct-2 DNA-binding domain using monoclonal antibodies*
**作者**: Gupta, S., et al.
**摘要**: 报道了一种高特异性Oct-2单克隆抗体的开发，通过X射线晶体学揭示了Oct-2蛋白与DNA结合的分子机制，为靶向药物设计提供依据。
4. **文献名称**: *Oct-2 as a biomarker in lymphoma: Immunohistochemical analysis*
**作者**: Tanaka, K., et al.
**摘要**: 利用Oct-2抗体对淋巴瘤组织样本进行免疫组化分析，发现Oct-2高表达与B细胞淋巴瘤的恶性程度及预后不良显著相关。
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注：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed)查询真实发表的论文。
The 2-Oct antibody, often associated with the transcription factor Oct-2. is a protein of interest in molecular biology and immunology. Oct-2 belongs to the POU (Pit-Oct-Unc) domain family of transcription factors, which regulate gene expression by binding to specific DNA sequences. Oct-2 is predominantly expressed in B lymphocytes and neuronal cells, where it plays a critical role in B-cell development, differentiation, and immunoglobulin gene regulation. Its interaction with coactivators like BOB.1 (OBF-1) enhances transcriptional activation, particularly at immunoglobulin promoters, making it essential for adaptive immunity.
In research, the 2-Oct antibody is widely used to detect Oct-2 protein levels in experimental models, aiding studies on B-cell malignancies, autoimmune disorders, and neurological diseases. Aberrant Oct-2 expression has been linked to conditions such as systemic lupus erythematosus (SLE) and certain B-cell lymphomas. The antibody’s applications include Western blotting, immunohistochemistry, and flow cytometry, providing insights into cellular localization and expression dynamics. Additionally, Oct-2’s role in neuronal gene regulation has spurred investigations into neurodegenerative diseases. Despite its specificity, cross-reactivity with related POU proteins (e.g., Oct-1) requires careful validation. Overall, the 2-Oct antibody remains a vital tool for dissecting transcriptional mechanisms in immunity and neurobiology.
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