| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IBA1; IRT1; AIF-1; IRT-1;;IBA1 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human IBA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Iba1抗体的经典文献概览：
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1. **文献名称**: *"A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage"*
**作者**: Imai, Y., Kohsaka, S.
**摘要**: 该研究首次克隆并鉴定了Iba1蛋白基因，证明其在单核细胞系(尤其是小胶质细胞)中特异性表达，并发现其通过结合肌动蛋白参与细胞形态变化，奠定了Iba1作为小胶质细胞标记物的基础(发表于*Biochemical and Biophysical Research Communications*, 1996)。
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2. **文献名称**: *"Iba1 is an actin-cross-linking protein in macrophages/microglia"*
**作者**: Ohsawa, K., Imai, Y., et al.
**摘要**: 研究揭示了Iba1蛋白通过EF-hand结构域结合钙离子，调控小胶质细胞/巨噬细胞中肌动蛋白的聚合与迁移功能，证实其在神经炎症和损伤修复中的关键作用(发表于*Biochemical and Biophysical Research Communications*, 2000)。
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3. **文献名称**: *"Microglial activation and chronic neurodegeneration"*
**作者**: Graeber, M.B., Streit, W.J.
**摘要**: 综述中重点讨论了Iba1抗体在神经退行性疾病(如阿尔茨海默病)研究中的应用，强调其作为小胶质细胞激活标志物在检测神经炎症动态变化中的重要性(发表于*Neurotherapeutics*, 2010)。
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**备注**：以上文献为Iba1抗体研究的基石，涵盖其分子机制、功能验证及临床应用。如需具体实验细节或最新进展，建议通过PubMed或Google Scholar检索近年研究。
Iba1 (Ionized calcium-binding adapter molecule 1) is a 17-kDa protein encoded by the *AIF1* (allograft inflammatory factor 1) gene, primarily expressed in microglia and macrophages. Discovered in the 1990s, Iba1 plays a critical role in regulating actin cytoskeleton dynamics, facilitating cell migration, phagocytosis, and membrane ruffling during immune responses. The Iba1 antibody, a widely used immunohistochemical marker, specifically targets this protein, enabling the identification and visualization of microglia in the central nervous system (CNS) and macrophages in peripheral tissues.
Its significance stems from microglia's dual role as CNS immune sentinels and contributors to neuroinflammation in diseases like Alzheimer’s, Parkinson’s, and stroke. Iba1 immunostaining reveals microglial activation states—transitioning from ramified (resting) to amoeboid (activated) morphology—providing insights into neuroinflammatory processes. Unlike other markers (e.g., CD68 for phagocytic activity), Iba1 labels both resting and activated microglia, making it a versatile tool for studying temporal and spatial changes in neuropathology. The antibody is validated across species, including humans, mice, and rats, and is compatible with techniques like immunofluorescence, Western blotting, and flow cytometry. Recent studies also explore its utility in tracking macrophage infiltration in tumors and injuries. Despite its widespread use, researchers must consider context-dependent expression variations and combine Iba1 with functional markers to fully interpret microglial behavior in health and disease.
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