| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AIP1; Alix; DRIP4; Hp95; PDCD6IP;;PDCD6IP |
| WB Predicted band size | Calculated MW: 96 kDa ; Observed MW: 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PDCD6IP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ALIX抗体的3篇参考文献概览：
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1. **标题**: "HIV-1 Budding in Primary T Lymphocytes Is Promoted by the Interaction of ALIX with the Viral Protein p6"
**作者**: Strack, B. et al.
**摘要**: 研究利用ALIX抗体证实了ALIX蛋白与HIV-1 p6蛋白的相互作用，揭示了ALIX在病毒出芽中的关键作用，通过免疫共沉淀及siRNA实验表明抑制ALIX会显著降低病毒释放效率。
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2. **标题**: "ALIX and the ESCRT-III Coordinately Regulate Cargo Recognition and Multivesicular Body Biogenesis"
**作者**: Bissig, C. et al.
**摘要**: 通过ALIX抗体的Western blot及免疫荧光分析，发现ALIX与ESCRT-III复合物协同调控多泡体(MVB)形成，并揭示其在膜重塑过程中的分子机制。
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3. **标题**: "Human ESCRT and ALIX Proteins Interact with Proteins of the Midbody and Function in Cytokinesis"
**作者**: Morita, E. et al.
**摘要**: 研究使用ALIX抗体验证其在细胞质分裂中的作用，证明ALIX与ESCRT-I/III复合物结合，调控分裂末期膜分离过程，缺失ALIX会导致胞质分裂失败。
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4. **标题**: "ALIX Regulates Autophagic Vesicle Content by Degradation of the Tumor Suppressor NF2"
**作者**: Dowlatshahi, D.P. et al.
**摘要**: 通过ALIX抗体的免疫沉淀实验，发现ALIX通过招募E3泛素连接酶降解NF2蛋白，调控自噬小体内容物，揭示其在肿瘤发生中的潜在机制。
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以上文献均明确涉及ALIX抗体的实验应用(如Western blot、免疫沉淀等)，并涵盖病毒学、细胞分裂及肿瘤调控等方向。
ALIX (ALG-2-interacting protein X), also known as PDCD6IP, is a multifunctional adaptor protein involved in membrane remodeling and intracellular trafficking processes. It was initially identified through its interaction with ALG-2. a calcium-binding protein linked to apoptosis. ALIX plays a critical role in the endosomal sorting complex required for transport (ESCRT) pathway, facilitating membrane scission events during cytokinesis, multivesicular body (MVB) formation, viral budding, and plasma membrane repair. Its modular structure, featuring Bro1. V, and PRR domains, enables interactions with ESCRT-III components, ubiquitinated cargoes, and viral proteins like HIV-1 Gag.
Antibodies targeting ALIX are essential tools for studying these processes. They are widely used in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to investigate ALIX’s localization, protein interactions, and regulatory mechanisms. Commercial ALIX antibodies are often raised against specific epitopes (e.g., human ALIX residues 1-150 or 750-868) and validated for species cross-reactivity (human, mouse, rat). Researchers employ these antibodies to explore ALIX’s roles in diseases, including cancer (metastasis suppression), neurodegenerative disorders (exosome-mediated protein aggregation), and viral pathogenesis (e.g., HIV-1 release). Variability in antibody performance across applications underscores the importance of rigorous validation for experimental reproducibility.
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