| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARX; SAE2; UBA2; UBLE1B;;UBA2 |
| WB Predicted band size | Calculated MW: 71 kDa ; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human UBA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于SAE2/UBA2抗体的参考文献示例(内容基于典型研究方向，部分为示例性概括)：
1. **"SUMOylation in Cancer: Mechanisms and Therapeutic Implications"**
- **作者**: Huang et al.
- **摘要**: 研究SUMO修饰通路在肿瘤发生中的作用，利用SAE2抗体检测癌细胞中SAE2蛋白表达水平，发现其高表达与预后不良相关，提示SAE2可能成为治疗靶点。
2. **"Structural Insights into the SUMO Activation Enzyme Heterodimer SAE1/UBA2"**
- **作者**: Lois et al.
- **摘要**: 通过X射线晶体学解析SAE1/SAE2(UBA2)复合体结构，使用特异性UBA2抗体验证蛋白相互作用，阐明其催化SUMO激活的分子机制。
3. **"Development of a Monoclonal Antibody Against SAE2 for Functional Proteomics Analysis"**
- **作者**: Hayashi et al.
- **摘要**: 报道一种高特异性抗SAE2单克隆抗体的开发，经Western blot和免疫荧光验证，成功应用于研究SAE2在细胞周期中的亚定位变化。
4. **"Dysregulation of SUMOylation in Neurodegenerative Diseases"**
- **作者**: Wilkinson et al.
- **摘要**: 分析阿尔茨海默病模型中SUMO通路异常，采用UBA2抗体检测脑组织中SAE2蛋白水平，揭示其表达下降可能导致神经元功能紊乱。
**注意**：以上文献为示例性内容，实际引用需根据具体研究检索数据库(如PubMed)。建议通过关键词“SAE2 antibody”或“UBA2 SUMO”查找最新论文。
The SAE2/UBA2 antibody targets a critical component of the SUMOylation machinery, a post-translational modification system analogous to ubiquitination. SAE2 (SUMO-activating enzyme subunit 2), also known as UBA2. forms a heterodimer with SAE1 to constitute the E1-activating enzyme responsible for initiating SUMO (Small Ubiquitin-like Modifier) conjugation. This enzyme activates SUMO proteins via ATP-dependent adenylation, enabling their transfer to E2-conjugating enzymes and subsequent attachment to substrate proteins. SUMOylation regulates diverse cellular processes, including transcription, DNA repair, nuclear transport, and stress responses.
SAE2/UBA2 antibodies are widely used in research to study SUMOylation dynamics in diseases such as cancer, neurodegenerative disorders, and viral infections, where aberrant SUMO modification is implicated. They enable detection of SAE2/UBA2 expression levels, localization (primarily nuclear), and interactions via techniques like Western blot, immunofluorescence, and immunoprecipitation. Additionally, these antibodies aid in exploring therapeutic strategies targeting the SUMO pathway, as inhibitors of SAE2/UBA2 are investigated for anticancer and antiviral applications. Their specificity and reliability make them essential tools for dissecting SUMO-mediated regulatory mechanisms in cellular homeostasis and pathology.
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