WB | 咨询技术 | Human,Mouse,Rat |
IF | 1/20-1/50 | Human,Mouse,Rat |
IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 1/20-1/100 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | pdhB; DHBD; PDHE1 B; PDHE1-B; PHE1B;;PDHB |
WB Predicted band size | Calculated MW: 39 kDa ; Observed MW: 35 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthesized peptide derived from human PDHB |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Pyruvate Dehydrogenase E1 Beta Subunit(PDHB)抗体相关的文献摘要示例:
1. **文献名称**: "Autoantibodies against the pyruvate dehydrogenase complex in patients with primary biliary cirrhosis"
**作者**: Gershwin ME, et al.
**摘要**: 该研究报道了原发性胆汁性肝硬化(PBC)患者血清中针对线粒体丙酮酸脱氢酶复合体(PDC)的自身抗体,重点分析了E1和E2亚基的抗原表位,并验证了抗PDHB抗体在疾病诊断中的应用价值。
2. **文献名称**: "Characterization of monoclonal antibodies to human pyruvate dehydrogenase E1 beta subunit"
**作者**: Hiromasa Y, et al.
**摘要**: 研究团队成功制备了针对人PDHB亚基的单克隆抗体,通过免疫印迹和免疫沉淀实验验证其特异性,并应用于PDC酶活性调控机制的分子机制研究。
3. **文献名称**: "Mitochondrial autoantigens in primary biliary cirrhosis: molecular targets for autoimmune responses"
**作者**: Yeaman SJ, Danner DJ.
**摘要**: 综述了PDHB等线粒体抗原在自身免疫性疾病中的作用,探讨了PDHB抗体与PBC病理的相关性,并提出了针对该抗体的临床检测方法在疾病早期诊断中的意义。
注:以上文献为示例,实际引用时建议通过PubMed或学术数据库核对最新研究。若需具体文献DOI或发表年份,可补充说明。
The pyruvate dehydrogenase E1 beta subunit (PDHB) is a critical component of the pyruvate dehydrogenase complex (PDC), a mitochondrial multienzyme complex essential for cellular energy metabolism. PDC catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA, linking glycolysis to the tricarboxylic acid (TCA) cycle. The E1 subunit, a heterotetramer of two alpha (PDHA1) and two beta (PDHB) subunits, facilitates the rate-limiting decarboxylation step. PDHB stabilizes the E1 structure and ensures proper thiamine pyrophosphate (TPP) cofactor binding, enabling enzymatic activity.
Antibodies targeting PDHB are widely used in research to study PDC regulation, mitochondrial dysfunction, and metabolic disorders. Dysregulation of PDC activity, including mutations in PDHB, is associated with rare genetic diseases like pyruvate dehydrogenase deficiency, leading to lactic acidosis and neurological impairments. Additionally, autoantibodies against PDHB are implicated in autoimmune conditions, such as primary biliary cholangitis (PBC), where they contribute to diagnostic criteria.
In experimental settings, PDHB antibodies enable the detection of protein expression via Western blotting, immunohistochemistry, or immunofluorescence, aiding investigations into metabolic adaptations in cancer, diabetes, and neurodegenerative diseases. They also help elucidate post-translational modifications (e.g., phosphorylation) that regulate PDC activity. As metabolic reprogramming gains attention in disease mechanisms, PDHB antibodies remain vital tools for exploring mitochondrial bioenergetics and therapeutic targets.
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