| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BKSR1; dKsr; EK31; KSR; KSR1; RSU2; SR31; ;KSR1 |
| WB Predicted band size | Calculated MW: 102 kDa ; Observed MW: 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human KSR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于KSR1抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*KSR1 regulates Raf-1 activation in a kinase-independent manner*
**作者**：Roy F. et al.
**摘要**：该研究通过使用特异性KSR1抗体，证实KSR1作为支架蛋白通过非激酶依赖的方式促进Raf-1/MEK/ERK信号通路的激活。实验表明，KSR1敲除会抑制Ras驱动的肿瘤细胞增殖，抗体检测显示其在细胞质膜上的定位对信号传导至关重要。
2. **文献名称**：*Targeting KSR1-mediated metabolic reprogramming potentiates MAPK pathway inhibition in pancreatic cancer*
**作者**：Yuan C. et al.
**摘要**：研究利用KSR1抗体进行免疫印迹和免疫组化分析，发现KSR1在胰腺癌中高表达，并通过调控糖酵解代谢增强MAPK通路活性。抑制KSR1可提高MEK抑制剂疗效，提示其作为联合治疗靶点的潜力。
3. **文献名称**：*Structural basis for the recognition of KSR1 by the prolyl isomerase FKBP12*
**作者**：Rodriguez D.A. et al.
**摘要**：通过KSR1抗体辅助的免疫共沉淀技术，揭示了KSR1与FKBP12的相互作用依赖于特定的脯氨酸结构域。该结构研究为开发靶向KSR1-FKBP12复合物的小分子抑制剂提供了理论依据。
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以上文献涵盖了KSR1在信号传导、癌症代谢及结构互作中的功能研究，均通过KSR1抗体进行关键实验验证。如需具体期刊及年份，可进一步补充数据库检索。
The KSR1 (Kinase Suppressor of Ras 1) antibody is a critical tool for studying the Ras-MAPK signaling pathway, which regulates cell proliferation, differentiation, and survival. KSR1 is a scaffolding protein that facilitates signal transduction by assembling Raf, MEK, and ERK kinases into functional complexes, enhancing the efficiency of MAPK activation. Initially identified in genetic screens as a Ras pathway modulator, KSR1's role varies contextually—it can act as a tumor promoter or suppressor depending on cellular conditions, making it a focus in cancer research.
KSR1 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect KSR1 expression, localization, and interactions. These antibodies are typically raised against specific epitopes of human or murine KSR1. with common host species including rabbit and mouse. Validation often involves knockout cell lines to confirm specificity. Research applications span cancer biology, developmental studies, and investigations into drug resistance, particularly for therapies targeting the Ras pathway. The antibody's utility extends to exploring post-translational modifications (e.g., phosphorylation) that regulate KSR1's scaffolding activity and membrane association. Its conserved CA1 and CA2 domains, critical for membrane localization and kinase recruitment, are frequent targets for antibody design. Reliable KSR1 antibodies are essential for elucidating its dual roles in disease and normal physiology.
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