Rabbit Monoclonal PFKFB3 Antibody

The PFKFB3 (6-phosphofructo-2-kinase/fructose-2.6-biphosphatase 3) antibody is a crucial tool for studying the role of PFKFB3. a bifunctional enzyme central to regulating glycolytic flux. PFKFB3 catalyzes the synthesis and degradation of fructose-2.6-bisphosphate (Fru-2.6-BP), a potent allosteric activator of phosphofructokinase-1 (PFK-1), a rate-limiting enzyme in glycolysis. By modulating Fru-2.6-BP levels, PFKFB3 fine-tunes glycolytic activity, making it pivotal in cellular energy homeostasis, proliferation, and adaptation to hypoxia. Its overexpression is linked to pathological conditions, including cancer, where it supports the Warburg effect, as well as ischemic diseases and inflammatory disorders. PFKFB3 antibodies enable researchers to detect and quantify PFKFB3 protein expression in tissues or cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are essential for exploring PFKFB3's involvement in metabolic reprogramming, angiogenesis, and cell survival pathways. Recent studies also highlight PFKFB3 as a potential therapeutic target, driving demand for validated antibodies to assess its inhibition in preclinical models. Commercial PFKFB3 antibodies are typically raised against specific epitopes, with validation including knockout controls to ensure specificity. Their applications span basic research, drug development, and biomarker studies in metabolic and oncological fields.