| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADCAD1; MEF2; MEF2A; Myocyte enhancer factor 2A; RSRFC4; RSRFC9; ;MEF2A/MEF2C |
| WB Predicted band size | Calculated MW: 51,55 kDa ; Observed MW: 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MEF2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于MEF2A和MEF2C抗体的3篇参考文献摘要概括：
1. **"MEF2C regulates T cell receptor signaling during CD4+ T cell development"**
- **作者**: Arnold, C.Y. 等
- **摘要**: 研究通过MEF2C抗体进行染色质免疫沉淀(ChIP)和Western blot分析，揭示MEF2C在调控T细胞受体信号通路中的作用，影响CD4+ T细胞分化和自身免疫疾病机制。
2. **"Cooperative interactions between MEF2A and HDAC4 regulate cardiac hypertrophy"**
- **作者**: Naya, F.J. 等
- **摘要**: 利用MEF2A特异性抗体研究其与组蛋白去乙酰化酶HDAC4的相互作用，发现两者协同调控心肌细胞肥大反应，为心脏病理机制提供新见解。
3. **"MEF2C is required for cortical neurogenesis and dendritic morphogenesis"**
- **作者**: Mao, Z. 等
- **摘要**: 通过MEF2C抗体阻断实验，证明MEF2C在皮质神经元发育中的关键作用，包括调控树突形态发生及突触可塑性，与神经退行性疾病相关。
这些研究均通过抗体实验阐明了MEF2A/C在细胞分化、疾病机制中的分子功能。
MEF2A and MEF2C are members of the myocyte enhancer factor 2 (MEF2) family of transcription factors, which play critical roles in cellular differentiation, development, and survival across multiple tissues, including muscle, brain, and immune cells. These proteins contain a conserved MADS-box domain and a MEF2-specific domain, enabling DNA binding and interaction with co-regulators. MEF2A is broadly expressed in cardiac muscle, skeletal muscle, and neurons, regulating genes involved in muscle development and synaptic plasticity. MEF2C is essential during embryogenesis, particularly in cardiac morphogenesis and neural crest cell differentiation, with ongoing roles in adult neurogenesis and immune responses.
Antibodies targeting MEF2A and MEF2C are vital tools for studying their expression patterns, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to investigate their regulatory mechanisms in health and disease. For instance, MEF2C dysfunction is linked to neurodevelopmental disorders, while MEF2A mutations are associated with coronary artery disease. Researchers rely on these antibodies to validate knockout models, assess post-translational modifications (e.g., phosphorylation), and explore context-dependent roles in cellular stress responses. Specificity is confirmed through knockdown/knockout controls or epitope mapping, ensuring accurate detection across species like human, mouse, and rat. These reagents remain indispensable for dissecting MEF2-mediated transcriptional networks in developmental biology and pathophysiology.
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