首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素23(IL-23)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL-23含量。IL-23是一种细胞因子，在免疫系统中发挥重要作用。其与炎症、自身免疫性疾病密切相关，如银屑病、炎症性肠病等。研究机制显示，它可激活特定的T细胞亚群，促进炎症因子的产生，引发免疫反应，目前是药物研发的热门靶点。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于体外炎症模型研究、免疫通路调控实验或药物干预效果评估等科研领域，为探索IL - 23在神经免疫、肠道屏障功能或慢性炎症反应中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IL-23
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-94 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-95 | ||||
| EDTA plasma (n=4) | 98 | 94-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.1472.1122.1301.9981001.8511.7761.8141.682501.3081.2881.2981.166250.9030.8980.9010.76912.50.6210.6140.6180.4866.250.3750.3590.3670.2353.120.2360.2240.2300.09800.1340.1300.132 | ||||||
货期：3-5 working days
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