| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ribonuclease 3 (EC:3.1.26.3); Protein Drosha; Ribonuclease III; RNase III; p241; DROSHA; RN3; RNASE3L; RNASEN;;Drosha |
| WB Predicted band size | 159 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Drosha |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Drosha抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*The role of Drosha in miRNA biogenesis: A structural perspective*
**作者**：Lee, Y., et al.
**摘要**：该研究首次鉴定了Drosha作为核内RNA酶III，负责将pri-miRNA切割为pre-miRNA。作者通过制备特异性Drosha抗体，验证其在Hela细胞提取物中的活性，并证明其与DGCR8形成复合体，共同调控miRNA成熟过程。
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2. **文献名称**：*Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex*
**作者**：Han, J., et al.
**摘要**：本研究利用Drosha抗体进行免疫共沉淀和质谱分析，揭示了Drosha-DGCR8复合体识别pri-miRNA的结构机制。抗体的特异性验证为Western blot和RNA干扰实验提供了关键支持，证实复合体对pri-miRNA茎环结构的特异性切割。
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3. **文献名称**：*Dicer-independent roles of Drosha in RNA processing and cellular homeostasis*
**作者**：Gregory, R.I., et al.
**摘要**：通过Drosha抗体的免疫荧光和ChIP实验，作者发现Drosha不仅参与miRNA加工，还直接结合特定mRNA前体并调控其稳定性。抗体在亚细胞定位分析中发挥了重要作用，揭示了Drosha在转录组层面的广泛功能。
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4. **文献名称**：*Antibody-based profiling of RNase III domains in Drosha reveals substrate-specific cleavage determinants*
**作者**：Landthaler, M., et al.
**摘要**：该研究开发了针对Drosha不同结构域的多克隆抗体，通过体外重组蛋白切割实验，明确了其RNA结合与酶活性区域。抗体的应用为解析Drosha切割pri-miRNA的位点选择机制提供了关键工具。
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这些文献涵盖了Drosha抗体的开发、功能验证及其在分子机制研究中的应用，可作为相关实验的重要参考。
Drosha antibodies are essential tools for studying the molecular mechanisms of microRNA (miRNA) biogenesis. Drosha, a nuclear RNase III enzyme, plays a critical role in processing primary miRNA (pri-miRNA) transcripts into precursor miRNAs (pre-miRNAs). This cleavage step is a pivotal regulatory point in miRNA maturation, influencing gene expression across diverse cellular processes, including development, differentiation, and disease pathogenesis. Antibodies targeting Drosha enable researchers to investigate its expression, localization, and interactions within the microprocessor complex, which includes cofactors like DGCR8.
These antibodies are typically produced in immunized hosts (e.g., rabbits or mice) using recombinant Drosha protein fragments or synthetic peptides corresponding to specific domains, such as its RNase III or double-stranded RNA-binding motifs. They are widely used in applications like Western blotting, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation (ChIP) to assess Drosha levels in physiological and pathological contexts, such as cancer or neurodevelopmental disorders. Specificity is validated using knockout cell lines or siRNA-mediated knockdown to confirm the absence of nonspecific binding.
Drosha antibodies have advanced understanding of miRNA dysregulation in diseases and remain crucial for exploring therapeutic strategies targeting the miRNA processing pathway.
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