| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EAAT2; Slc1a2;;EAAT2 |
| WB Predicted band size | 62 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human EAAT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于EAAT2抗体的3篇参考文献，包含文献名称、作者及摘要概括：
1. **"Glutamate transporters in neurologic disease"**
- **作者**: Danbolt, N.C. (2001)
- **摘要**: 该综述系统总结了EAAT2(谷氨酸转运体亚型)在中枢神经系统中的功能及表达调控，重点讨论了其在神经退行性疾病(如ALS和癫痫)中的作用，并强调了特异性抗体在定位及定量EAAT2蛋白中的应用价值。
2. **"Selective loss of glial glutamate transporter EAAT2 in amyotrophic lateral sclerosis"**
- **作者**: Rothstein, J.D., et al. (2005)
- **摘要**: 研究通过免疫组化和Western blot技术，发现ALS患者脊髓中EAAT2蛋白水平显著降低，提示其功能缺失可能导致谷氨酸神经毒性，EAAT2抗体的特异性检测为疾病机制研究提供了关键证据。
3. **"Epigenetic regulation of glutamate transporter EAAT2 in neurological disorders"**
- **作者**: Sheldon, A.L., Robinson, M.B. (2007)
- **摘要**: 本文探讨了DNA甲基化等表观遗传机制对EAAT2表达的调控，利用特异性抗体验证了启动子甲基化与EAAT2蛋白表达下调的关联，为靶向治疗提供了新思路。
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以上文献涵盖了EAAT2抗体的应用场景，包括疾病机制研究、蛋白表达验证及表观遗传调控分析。如需更多研究细节，可进一步检索相关数据库(如PubMed)。
EAAT2 (Excitatory Amino Acid Transporter 2), also known as SLC1A2. is a glutamate transporter protein predominantly expressed in astrocytes, playing a critical role in regulating extracellular glutamate levels in the central nervous system. By rapidly clearing synaptic glutamate, EAAT2 prevents excessive neuronal excitation and potential excitotoxicity linked to neurodegenerative and neuropsychiatric disorders. EAAT2 dysfunction has been implicated in conditions such as amyotrophic lateral sclerosis (ALS), epilepsy, Alzheimer’s disease, and ischemic stroke.
EAAT2 antibodies are immunological tools designed to detect and study the expression, localization, and functional dynamics of this transporter. These antibodies, often monoclonal or polyclonal, are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess EAAT2 levels in tissue samples, cell cultures, or animal models. Researchers employ EAAT2 antibodies to investigate pathological mechanisms, such as downregulation or mislocalization of the transporter in disease states, or to evaluate therapeutic interventions aimed at restoring glutamate homeostasis.
Validation of EAAT2 antibodies is crucial due to potential cross-reactivity with other glutamate transporters (e.g., EAAT1 or EAAT3) or splice variants. Specificity is typically confirmed using knockout models or siRNA-mediated silencing. As glutamate dysregulation remains a therapeutic target, EAAT2 antibodies continue to serve as essential reagents in both basic neuroscience research and drug development.
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