| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HNRNPA 1; HNRNPA1; HNRPA1;;hnRNP A1 |
| WB Predicted band size | Calculated MW: 39 kDa ; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human hnRNP A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于hnRNPA1抗体的3-4篇代表性文献的简要示例(内容为模拟，建议通过数据库核实真实文献)：
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1. **文献名称**：*hnRNPA1 regulates mRNA splicing and stability in neuronal development*
**作者**：Smith J, et al.
**摘要**：研究利用hnRNPA1抗体进行免疫沉淀和Western blot分析，揭示hnRNPA1通过结合特定mRNA调控神经元发育中的剪接和稳定性，其缺失导致突触蛋白表达异常。
2. **文献名称**：*Pathogenic mutations in hnRNPA1 promote cytoplasmic aggregation and ALS pathogenesis*
**作者**：Kim HJ, Taylor JP.
**摘要**：通过免疫荧光和抗体标记，发现ALS相关hnRNPA1突变体在细胞质中形成病理性聚集，破坏RNA-蛋白相互作用，促进神经退行性变。
3. **文献名称**：*Antibody-based detection of hnRNPA1 in stress granules*
**作者**：Li S, et al.
**摘要**：开发特异性hnRNPA1单克隆抗体，验证其在应激颗粒中的共定位，证明hnRNPA1通过液-液相分离参与细胞应激反应，与TDP-43存在功能协同。
4. **文献名称**：*hnRNPA1 autoantibodies in autoimmune encephalitis*
**作者**：Lee KH, et al.
**摘要**：首次报道部分自身免疫性脑炎患者血清中存在抗hnRNPA1抗体，提示其可能通过干扰RNA代谢参与神经系统自身免疫病理过程。
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注：以上为模拟示例，实际文献请通过PubMed/Google Scholar检索关键词(如“hnRNPA1 antibody”、“hnRNPA1 function”、“hnRNPA1 disease”)。
Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) is a member of the hnRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, mRNA transport, stability, and translation regulation. It contains RNA-binding domains (RRMs) and a prion-like domain, enabling its involvement in dynamic ribonucleoprotein assembly. hnRNPA1 antibodies are essential tools for studying its expression, localization, and function in both physiological and pathological contexts.
Research highlights hnRNPA1's dual role in health and disease. It regulates telomere maintenance, DNA repair, and stress granule formation. However, mutations in its prion-like domain (e.g., D262V) are linked to neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) and multisystem proteinopathy, where hnRNPA1 forms pathological aggregates. hnRNPA1 antibodies help detect these aggregates in disease models.
In cancer, hnRNPA1 is often overexpressed and promotes tumor progression by modulating oncogenic mRNAs (e.g., MYC, KRAS). Antibodies enable its identification as a potential biomarker or therapeutic target. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate hnRNPA1's dysregulation in tissues or cell lines.
Overall, hnRNPA1 antibodies are pivotal in dissecting its contributions to RNA biology, neurodegeneration, and cancer, bridging molecular mechanisms with clinical insights.
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