WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 1/20-1/100 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; PAP-IV; Protein I; p36; ANXA2; ANX2; ANX2L4; CAL1H; LPC2D; Annexin2; HEL S 270;;Annexin A2 |
WB Predicted band size | Calculated MW: 39 kDa ; Observed MW: 38 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthesized peptide derived from human Annexin A2 |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Annexin A2抗体的代表性文献摘要(信息基于公开论文整理):
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1. **《Annexin A2: a mediator of cell surface plasmin generation》**
*作者:Hajjar KA, et al. (2004)*
摘要:综述Annexin A2作为细胞表面纤溶酶原受体复合物的关键组分,通过抗体阻断实验证实其促进纤溶酶生成,影响血管通透性和肿瘤转移的分子机制。
2. **《Annexin A2 antibodies in the diagnosis and prognosis of pancreatic cancer》**
*作者:Sharma MC, et al. (2011)*
摘要:研究Annexin A2在胰腺癌组织中的过表达,通过免疫组化(使用特异性抗体)发现其与患者生存率负相关,提示其作为潜在生物标志物的价值。
3. **《Annexin A2 interacts with the actin cytoskeleton and promotes HIV-1 infection in macrophages》**
*作者:Ryzhova E, et al. (2016)*
摘要:利用Annexin A2抗体进行免疫共沉淀和功能抑制实验,揭示其通过调节肌动蛋白重塑促进HIV-1病毒进入巨噬细胞的分子通路。
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注:如需具体文献全文,建议通过PubMed或Sci-Hub输入标题/作者查询DOI获取。
Annexin A2 (AnxA2) is a calcium-dependent phospholipid-binding protein belonging to the annexin superfamily, involved in diverse cellular processes including membrane organization, exocytosis, endocytosis, and cytoskeletal dynamics. It forms a heterotetrameric complex with S100A10 (p11), which facilitates interactions with actin, phospholipids, and other proteins to regulate cell adhesion, migration, and membrane repair. AnxA2 is implicated in pathological conditions such as cancer metastasis, thrombosis, and autoimmune diseases due to its roles in fibrinolysis, angiogenesis, and extracellular matrix remodeling.
Antibodies targeting Annexin A2 are essential tools for studying its expression, localization, and function. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Monoclonal antibodies (e.g., clone 5) offer high specificity for detecting AnxA2 in human or murine samples, while polyclonal antibodies may detect conserved epitopes across species. Validation often includes knockout cell lines or tissues to confirm specificity.
In research, AnxA2 antibodies help elucidate its dual roles in health and disease. For example, elevated AnxA2 expression in tumors correlates with metastatic potential, while autoantibodies against AnxA2 are linked to antiphospholipid syndrome. However, cross-reactivity with other annexins (e.g., AnxA1. AnxA5) requires careful antibody selection. Overall, AnxA2 antibodies remain critical for exploring its therapeutic potential and biomarker utility in cancer, cardiovascular disorders, and immune dysregulation.
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