| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Claudin5; Transmembrane protein deleted in VCFS; BEC1; CPETRL1; TMDVCF; CLDN5; AWAL; TMVCF;;Claudin 5 |
| WB Predicted band size | Calculated MW: 23 kDa ; Observed MW: 20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Claudin 5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Claudin5抗体的3篇参考文献及其摘要内容，信息基于公开研究整理：
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1. **文献名称**：*Claudin-5: A Novel Target for Tight Junction Modulation in Brain Endothelial Cells*
**作者**：Morita K, Sasaki H, Furuse M, et al.
**摘要**：本研究利用Claudin5抗体，通过免疫荧光和Western blot技术，验证了Claudin5在血脑屏障内皮细胞中的特异性表达。发现其缺失会导致血脑屏障通透性异常，提示Claudin5抗体可用于评估神经疾病模型中屏障完整性。
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2. **文献名称**：*Loss of Claudin-5 in Gliomas Promotes Tumor Cell Invasion via Dysregulated Matrix Metalloproteinases*
**作者**：Nitta T, Hata M, Gotoh S, et al.
**摘要**：通过Claudin5抗体免疫组化分析，发现胶质瘤组织中Claudin5表达降低与患者预后不良相关。实验表明Claudin5缺失通过激活MMPs促进肿瘤侵袭，其抗体可作为潜在诊断标志物。
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3. **文献名称**：*Antibody-based Disruption of Claudin-5 in Mouse Blood-Brain Barrier Models*
**作者**：Greene C, Hanley N, Campbell M.
**摘要**：研究开发了一种靶向Claudin5的单克隆抗体，在小鼠模型中证明其可短暂开放血脑屏障，增强化疗药物递送。该抗体为CNS药物递送系统开发提供了新策略。
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如需具体文献来源，建议通过PubMed或Google Scholar检索上述标题获取全文信息。
Claudin5 is a key component of tight junction complexes, primarily expressed in endothelial and epithelial cells, where it plays a critical role in regulating paracellular permeability and maintaining cellular polarity. As a member of the claudin family, it is integral to the formation of the blood-brain barrier (BBB), selectively restricting the passage of molecules between circulating blood and the central nervous system. Its structure includes four transmembrane domains, contributing to the selective "sealing" of intercellular spaces. Dysregulation of Claudin5 has been linked to various pathologies, including stroke, neurodegenerative diseases (e.g., Alzheimer's), and cancer metastasis, where BBB integrity is compromised or cellular invasion is promoted.
Antibodies targeting Claudin5 are essential tools in biomedical research, enabling the detection, localization, and functional analysis of this protein. They are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to study BBB dynamics in health and disease. For instance, reduced Claudin5 expression is often observed in neuroinflammatory conditions, while its overexpression in certain tumors correlates with aggressive behavior. Researchers also utilize these antibodies to explore therapeutic strategies aimed at modulating tight junction function—for example, enhancing drug delivery across the BBB or inhibiting cancer cell dissemination. Validated Claudin5 antibodies are critical for ensuring specificity in these applications, particularly given the structural similarities among claudin family members.
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