关键信息
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基因名
ASS1
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简介
ASS1 Protein, Human (His) is a recombinant human Argininosuccinate synthase expressed in E. coli with a His tag. Argininosuccinate synthase is an enzyme that catalyzes the synthesis of argininosuccinate from citrulline and aspartate[1].
- 应用
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别名
rHuASS1, His; Argininosuccinate Synthase; Citrulline--Aspartate Ligase; ASS1; ASS
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P00966
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表达区间
M1-K412
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氨基酸序列
HHHHHHMSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSREFVEEFIWPAIQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELSCYSLAPQIKVIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK
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蛋白长度
Full Length
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分子量
50.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ASS1 (argininosuccinate synthase 1) is an essential enzyme in the urea cycle, playing a critical role in the conversion of citrulline and aspartate into argininosuccinate, which is subsequently catabolized to produce arginine and fumarate. Mutations in the ASS1 gene can lead to argininosuccinic aciduria, a rare metabolic disorder characterized by hyperammonemia and various neurological impairments. Given its importance in metabolism and the implications of ASS1-related disorders, research on recombinant ASS1 protein has garnered significant attention. The recombinant expression of ASS1 allows for the detailed study of its enzymatic properties, structure-function relationships, and potential therapeutic applications. By producing large quantities of the protein, researchers can investigate the effects of specific mutations, identify potential inhibitors or activators, and explore its role in other physiological processes. Moreover, recombinant ASS1 can serve as a valuable tool in gene therapy approaches, aiming to restore normal metabolic function in affected individuals. This research ultimately contributes to our understanding of metabolic diseases and aids in the development of targeted treatments.
