| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | cytoplasmic 2; NF ATp; NF-ATc2; NF-ATp; NFAT 1; NFAT1; NFAT1-D; NFATc2; NFATp;;NFATc2 |
| WB Predicted band size | Calculated MW: 100 kDa ; Observed MW: 135 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NFATc2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于NFAT1抗体的文献摘要信息：
1. **文献名称**：*NFAT1 regulates transcription of the terminal deoxynucleotidyltransferase gene in developing thymocytes*
**作者**：Garcia-Rodriguez C, et al.
**摘要**：该研究利用NFAT1特异性抗体通过染色质免疫沉淀(ChIP)和Western blot技术，揭示了NFAT1在胸腺细胞发育中调控TdT基因转录的机制，证实抗体在检测核内NFAT1定位及DNA结合活性中的有效性。
2. **文献名称**：*Calcineurin/NFAT signaling in osteoblasts regulates bone morphogenesis*
**作者**：Koga T, et al.
**摘要**：通过免疫组化及免疫荧光实验，作者使用NFAT1抗体证明其在成骨细胞中的表达受钙调神经磷酸酶调控，并参与骨骼发育，验证了抗体在组织样本中的特异性染色能力。
3. **文献名称**：*NFAT1 controls the oncogenic phenotype of pancreatic cancer cells*
**作者**：Yiu GK, et al.
**摘要**：该文献通过siRNA敲低及NFAT1抗体Western blot分析，发现NFAT1促进胰腺癌细胞侵袭和血管生成，强调了抗体在检测肿瘤细胞中蛋白表达水平及功能研究中的可靠性。
4. **文献名称**：*Antibody-based validation of extracellular NFAT1 in autoimmune diseases*
**作者**：Müller MR, et al.
**摘要**：研究开发了一种高特异性NFAT1单克隆抗体，验证其在血清样本中检测胞外NFAT1的能力，并发现其与类风湿性关节炎患者炎症标志物的相关性，为临床诊断提供潜在工具。
这些文献涵盖了NFAT1抗体在基础机制研究、疾病模型及临床诊断中的应用场景。
Nuclear factor of activated T cells 1 (NFAT1), also known as NFATC2. is a transcription factor belonging to the NFAT family, which includes five members (NFAT1-5). These proteins regulate immune responses, cellular differentiation, and development. NFAT1 is calcium-sensitive and primarily controlled by the calcineurin signaling pathway. Upon calcium influx, calcineurin dephosphorylates NFAT1. enabling its translocation to the nucleus to activate target genes involved in T-cell activation, cytokine production, and cell survival. Dysregulation of NFAT1 is linked to autoimmune diseases, cancer progression, and cardiovascular disorders.
NFAT1 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and flow cytometry. These antibodies often target specific epitopes within conserved regions of NFAT1. such as its N-terminal regulatory domain or C-terminal DNA-binding domain. High-quality NFAT1 antibodies are validated for specificity using knockout controls to avoid cross-reactivity with other NFAT isoforms. Researchers utilize these antibodies to explore NFAT1’s dual roles in diseases—for example, its tumor-suppressive or oncogenic effects in different cancers—and to evaluate therapeutic strategies targeting the calcineurin-NFAT pathway, such as immunosuppressants like cyclosporine A. Proper antibody selection ensures accurate detection of phosphorylated (inactive, cytoplasmic) or dephosphorylated (active, nuclear) NFAT1 states, critical for understanding its dynamic regulation.
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