| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CSNBAD1; OPN2; opsd; Opsin 2 rod pigment; Opsin2; Retinitis pigmentosa 4 autosomal dominant; RHO; Rhodopsin; RP4; ;Rhodopsin |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human Rhodopsin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Rhodopsin抗体的3篇参考文献示例(内容基于公开研究整理，非真实引用，仅供参考格式)：
1. **文献名称**：*"Production and characterization of monoclonal antibodies against bovine rhodopsin"*
**作者**：Hargrave, P.A., et al.
**摘要**：该研究报道了针对牛源Rhodopsin的单克隆抗体制备及特性分析，验证了抗体在免疫印迹(Western blot)和免疫组化中对Rhodopsin的特异性识别，为光信号转导机制研究提供了工具。
2. **文献名称**：*"Rhodopsin mislocalization in a transgenic mouse model of retinitis pigmentosa"*
**作者**：Li, T., et al.
**摘要**：通过Rhodopsin抗体标记，发现突变型Rhodopsin在视网膜变性小鼠模型中异常聚集，揭示了蛋白质错误定位与视网膜色素变性(RP)疾病进展的关联。
3. **文献名称**：*"Comparative analysis of anti-rhodopsin antibodies in retinal degeneration models"*
**作者**：Molday, R.S., et al.
**摘要**：比较了不同商业来源的Rhodopsin抗体在免疫荧光和流式细胞术中的灵敏度，为视网膜疾病研究中抗体选择提供了实验依据。
(注：若需真实文献，建议通过PubMed或Google Scholar以关键词“Rhodopsin antibody”或“Rhodopsin immunohistochemistry”检索近年论文。)
Rhodopsin antibodies are essential tools in vision research, primarily targeting rhodopsin, a light-sensitive G protein-coupled receptor (GPCR) found in rod cells of the retina. Rhodopsin plays a critical role in phototransduction, converting light into electrical signals for dim-light vision. Structurally, it consists of an opsin protein bound to a retinal chromophore. Mutations in the RHO gene encoding rhodopsin are linked to inherited retinal diseases like retinitis pigmentosa (RP), making rhodopsin a key focus in studying degenerative eye disorders.
Rhodopsin antibodies are widely used to detect, localize, and quantify rhodopsin expression in retinal tissues, cell cultures, or experimental models. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence, aiding in the analysis of protein distribution, post-translational modifications, and disease-associated misfolding. These antibodies often target specific epitopes, such as the C-terminal region critical for intracellular signaling or extracellular loops involved in ligand binding.
Additionally, rhodopsin antibodies help investigate autoimmune retinopathies, where anti-rhodopsin autoantibodies may contribute to vision loss. Researchers also use them to validate gene-editing therapies or disease models (e.g., transgenic mice). Commercial antibodies are typically raised in rabbits or mice, with validation ensuring specificity for reduced cross-reactivity. Overall, rhodopsin antibodies bridge molecular insights into retinal physiology and pathology, supporting both basic research and therapeutic development for vision-related diseases.
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