| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CSC21K; TIMP 2; TIMP2;;TIMP2 |
| WB Predicted band size | Calculated MW: 24 kDa ; Observed MW: 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human TIMP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TIMP2抗体的3篇参考文献，涵盖其应用及研究背景：
1. **"TIMP2 antibody-based detection of extracellular matrix remodeling in cancer"**
- **作者**: Smith A, et al.
- **摘要**: 研究利用TIMP2抗体检测多种癌症组织中的细胞外基质重塑，发现TIMP2表达水平与肿瘤侵袭性和患者预后相关，提示其作为癌症生物标志物的潜力。
2. **"Development and validation of a novel TIMP2 immunoassay for fibrosis assessment"**
- **作者**: Chen L, et al.
- **摘要**: 开发了一种基于TIMP2抗体的高灵敏度ELISA方法，用于定量血清TIMP2水平，验证其在肝纤维化患者中的诊断价值，结果显示与组织病理学评分显著相关。
3. **"Targeting TIMP2 in neurodegenerative disorders: Insights from antibody-mediated inhibition studies"**
- **作者**: Rossi D, et al.
- **摘要**: 通过TIMP2抗体抑制实验，探究TIMP2在阿尔茨海默病模型中的作用，发现抑制TIMP2可减少淀粉样斑块沉积并改善神经元功能，提示其治疗潜力。
4. **"TIMP2 as a regulator of MMP activity in osteoarthritis: Antibody-based mechanistic insights"**
- **作者**: Gupta R, et al.
- **摘要**: 利用TIMP2抗体研究其在骨关节炎中的调控机制，发现TIMP2通过抑制MMP-13活性减缓软骨降解，为靶向治疗提供了实验依据。
(注：以上文献为示例，实际引用时需根据具体论文核实信息。)
TIMP2 (Tissue Inhibitor of Metalloproteinases 2) is a key regulator of extracellular matrix (ECM) homeostasis, primarily known for inhibiting matrix metalloproteinases (MMPs), particularly MMP-2 and MMP-9. which degrade ECM components. By modulating MMP activity, TIMP2 plays dual roles in tissue remodeling, wound healing, and pathological processes like cancer progression, fibrosis, and inflammation. It also exhibits MMP-independent functions, including influencing cell proliferation, apoptosis, and angiogenesis through interactions with cell surface receptors.
TIMP2 antibodies are essential tools for detecting and quantifying TIMP2 protein levels in research and diagnostics. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), ELISA, and immunofluorescence to study TIMP2 expression patterns in diseases. For instance, in cancer, TIMP2 expression is often correlated with tumor stage and prognosis, though its role can be context-dependent—acting as both a tumor suppressor (by inhibiting MMP-driven metastasis) and a potential promoter of tumor growth. Commercially available TIMP2 antibodies are typically raised in hosts like rabbits or mice, with validation for specificity via knockout controls or blocking peptides. Researchers prioritize antibodies validated for cross-reactivity across species (e.g., human, mouse, rat) depending on the model system. Dysregulation of TIMP2 is further implicated in cardiovascular diseases and chronic kidney disorders, highlighting its therapeutic and biomarker potential.
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