| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cytochrome P450 2E1; CYPIIE1; Cytochrome P450-J; CYP2E; P450C2E; CYPIIE1;;Cytochrome P450 2E1 |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Cytochrome P450 2E1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CYP2E1抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**: "Role of CYP2E1 in the pathogenesis of alcoholic liver disease: mechanisms and therapeutic potential"
**作者**: Lu Y, Cederbaum AI
**摘要**: 该研究探讨CYP2E1在酒精性肝病中的作用，通过免疫组化和Western blot分析发现，CYP2E1在酒精暴露的小鼠肝脏中表达显著升高，其抗体特异性验证表明与氧化应激和脂质过氧化密切相关，提示CYP2E1可能是治疗靶点。
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2. **文献名称**: "Comparative analysis of CYP2E1 antibody specificity in human and rodent tissues"
**作者**: Novak RF, Woodcroft KJ
**摘要**: 研究比较了不同商业来源的CYP2E1抗体在人和啮齿动物组织中的交叉反应性，发现部分抗体存在非特异性结合问题，强调选择高特异性抗体对精准检测CYP2E1表达至关重要。
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3. **文献名称**: "CYP2E1-mediated drug toxicity: insights from antibody-based detection in hepatic models"
**作者**: Gonzalez FJ, Kim D
**摘要**: 利用CYP2E1抗体研究药物代谢诱导的肝毒性机制，发现CYP2E1的过表达通过激活炎症通路加剧肝细胞损伤，抗体检测结果为药物开发中的毒性评估提供了实验依据。
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如需具体文献来源，可通过PubMed或Google Scholar检索标题或作者获取全文。
CYP2E1 (Cytochrome P450 2E1) is a member of the cytochrome P450 enzyme superfamily, primarily expressed in the liver, and plays a critical role in metabolizing exogenous substances (e.g., ethanol, acetaminophen, and industrial solvents) and endogenous compounds (e.g., fatty acids). It is notable for its induction by alcohol and fasting, contributing to oxidative stress through the generation of reactive oxygen species (ROS) during substrate oxidation. This enzyme is implicated in drug-induced hepatotoxicity, alcoholic liver disease, and metabolic disorders.
CYP2E1 antibodies are essential tools in biomedical research for detecting and quantifying CYP2E1 protein expression in tissues or cell lines. They are widely used in techniques like Western blotting, immunohistochemistry, and ELISA to study enzyme regulation, tissue-specific expression, and interactions with drugs or toxins. Researchers employ these antibodies to investigate CYP2E1's role in diseases such as non-alcoholic fatty liver disease (NAFLD), cancer (due to its activation of procarcinogens), and metabolic syndromes.
The development of CYP2E1 antibodies has advanced understanding of its polymorphic variants, transcriptional regulation (e.g., by HNF-1α and PPARs), and post-translational modifications. Challenges remain in distinguishing CYP2E1 from closely related isoforms, emphasizing the need for highly specific antibodies. Overall, these reagents are pivotal in elucidating CYP2E1's dual role in detoxification and pathological processes.
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