首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素19(IL-19)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL19含量。IL19是一种白细胞介素-10家族的细胞因子，具有多方面的生物学功能。其研究机制涉及调节表皮功能和炎症反应，与多种疾病如炎症性肠病、衰老、糖尿病等密切相关。IL19在胶质母细胞瘤治疗中显示出作为新兴靶点的潜力，其抗体疗法与化疗结合有望改善治疗效果。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品特别适用于研究炎症性疾病模型、免疫调控机制以及药物干预实验中IL - 19的动态变化，为探索其在慢性呼吸系统疾病、皮肤屏障功能异常及肿瘤微环境调控中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL 10C ELISA Kit; IL 19 ELISA Kit; IL-19 ELISA Kit; Il19 ELISA Kit; IL19_HUMAN ELISA Kit; Interleukin 19 ELISA Kit; Interleukin-19 ELISA Kit; MDA1 ELISA Kit; Melanoma differentiation-associated protein-like protein ELISA Kit; NG.1 ELISA Kit; ZMDA1 ELISA Kit
缩写：IL19
Uniprot No.：Q9UHD0
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 100-110 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
回收率：
| The recovery of human IL-19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 80-89 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.5722.4362.5042.3985001.8311.7251.7781.6722500.9851.0030.9940.8881250.5490.5570.5530.44762.50.3270.3120.3200.21431.250.2160.2040.2100.10415.60.1600.1680.1640.05800.1030.1090.106 | |||||||
货期：3-5 working days
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