| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADP ribosylation factor 6; ARF6; Small GTP binding protein;;ADP ribosylation factor 6 |
| WB Predicted band size | 20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ADP ribosylation factor 6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ARF6抗体的3篇代表性文献，简要总结其研究内容：
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1. **文献名称**：*ARF6 regulates tumor invasion and metastasis through the control of endocytic membrane trafficking*
**作者**：D'Souza-Schorey C, et al.
**摘要**：研究揭示了ARF6在肿瘤侵袭和转移中的关键作用，通过调控细胞膜运输和细胞骨架重组促进癌细胞迁移。研究中采用ARF6特异性抗体进行免疫荧光定位及Western blot验证敲低效率。
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2. **文献名称**：*ARF6 modulates recycling of E-cadherin and impacts epithelial-mesenchymal transition*
**作者**：Palacios F, et al.
**摘要**：本文发现ARF6通过调节E-cadherin的膜循环影响上皮-间质转化(EMT)。实验利用ARF6抗体进行免疫沉淀和共聚焦成像，证实其与细胞黏附复合物的动态关联。
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3. **文献名称**：*ARF6 GTPase signaling in neuronal morphogenesis and synaptic plasticity*
**作者**：Hern-Ández-Muñoz I, et al.
**摘要**：探讨ARF6在神经元形态发生和突触可塑性中的功能，通过抗体介导的ARF6活性检测(如免疫印迹和免疫组化)，证明其调控突触囊泡运输的分子机制。
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4. **文献名称**：*ARF6-dependent activation of ERM proteins mediates cytoskeletal remodeling*
**作者**：Santy LC, et al.
**摘要**：研究ARF6通过激活ERM蛋白(ezrin/radixin/moesin)调控细胞骨架重组的分子通路。实验中ARF6抗体用于检测其与细胞膜微结构域的共定位及活性状态变化。
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**备注**：以上文献为示例，实际引用时建议核对PubMed或期刊数据库获取准确信息。若需具体实验用途(如抗体品牌、克隆号)，可补充说明进一步筛选。
The ARF6 (ADP-ribosylation factor 6) antibody is a crucial tool for studying the function and regulation of ARF6. a small GTPase belonging to the ARF family. ARF6 plays a central role in membrane trafficking, cytoskeletal reorganization, and cellular signaling. It cycles between active GTP-bound and inactive GDP-bound states, interacting with effector proteins like PI4K, PLD, and ARNO to regulate endocytic recycling, exocytosis, and actin dynamics. Dysregulation of ARF6 is implicated in cancer metastasis, neuronal development, and immune responses, making it a target for biomedical research.
ARF6 antibodies are designed to detect endogenous ARF6 protein in various experimental applications, including Western blotting, immunofluorescence, and immunoprecipitation. These antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions of human ARF6. and validated for cross-reactivity across species (e.g., human, mouse, rat). Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect broader isoforms but require rigorous validation. Researchers rely on ARF6 antibodies to investigate its subcellular localization, expression levels under pathological conditions (e.g., tumor samples), and interactions with regulatory proteins like GAPs and GEFs. Commercial suppliers (e.g., Santa Cruz, Abcam) often provide validation data using knockout cell lines or siRNA knockdown to confirm antibody specificity. Proper controls, such as peptide blocking experiments, are essential to minimize off-target effects in ARF6-related studies.
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