| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Parvalbumin alpha; PVALB;;Parvalbumin alpha |
| WB Predicted band size | 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Parvalbumin alpha |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于Parvalbumin(PV)抗体的代表性文献，包含名称、作者及摘要概括：
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1. **"Parvalbumin in most gamma-aminobutyric acid-containing neurons of the rat cerebral cortex"**
*Celio, M.R.* (1990)
**摘要**：本研究利用PV抗体，通过免疫组织化学技术系统揭示了PV蛋白在成年大鼠大脑皮层GABA能中间神经元中的广泛分布，表明PV是此类神经元的关键分子标记，并探讨了其与神经元电生理特性的关联。
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2. **"A developmental switch in the response of GABAergic neurons to parvalbumin-specific antibodies"**
*Hippenmeyer, S. et al.* (2005)
**摘要**：文章通过PV抗体的标记，追踪了小鼠大脑皮层中间神经元在发育过程中的分化与迁移，发现PV表达在特定发育阶段激活，提示其与神经元成熟及功能整合密切相关。
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3. **"Physiological subgroups of nonpyramidal cells in rat frontal cortex revealed by cluster analysis of parvalbumin immunoreactivity"**
*Kawaguchi, Y. & Kubota, Y.* (1995)
**摘要**：结合电生理记录与PV抗体标记，作者对大鼠前额叶皮层非锥体神经元进行分类，提出PV阳性神经元具有独特的快速放电特性，为GABA能神经元亚型的功能研究奠定基础。
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4. **"Optogenetic dissection of cortical microcircuits mediated by parvalbumin-expressing interneurons"**
*Yizhar, O. et al.* (2011)
**摘要**：利用PV抗体鉴定转基因小鼠的中间神经元类型，结合光遗传学技术解析PV阳性神经元在皮层微环路中的抑制作用，揭示其在信息处理及疾病模型中的关键角色。
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这些文献涵盖了PV抗体在神经解剖学、发育生物学及功能环路研究中的典型应用，展示了其在标记特定神经元亚群及解析其功能中的重要性。
Parvalbumin (PV) is a calcium-binding protein belonging to the EF-hand protein family, widely expressed in the nervous system, muscle tissue, and endocrine cells. First identified in fish muscle, it plays a critical role in calcium buffering and signaling, particularly in fast-twitch muscle fibers and GABAergic interneurons in the brain. In the central nervous system, PV is a marker for a subset of inhibitory interneurons that regulate network oscillations and synaptic plasticity. These fast-spiking PV+ neurons are essential for maintaining excitatory-inhibitory balance and are implicated in cognitive functions, including memory and sensory processing.
PV antibodies are vital tools for identifying and studying these specialized neurons. Generated through immunization with purified PV protein or peptide fragments, these antibodies enable visualization of PV-expressing cells via immunohistochemistry, immunofluorescence, and Western blotting. Their specificity is crucial for mapping neuronal circuits and investigating PV's role in diseases such as epilepsy, schizophrenia, and autism, where PV+ neuron dysfunction is observed. Additionally, PV antibodies aid in studying muscle disorders linked to calcium dysregulation. Recent advances in antibody validation techniques, including knockout controls, have enhanced their reliability in both research and diagnostic applications, solidifying their importance in neuroscience and beyond.
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