| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ago 2; Argonaute 2; dAgo2; eIF2C 2; hAgo2; PPD; ;Argonaute 2 |
| WB Predicted band size | 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Argonaute 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Argonaute2抗体的3-4篇参考文献的简要列举：
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1. **文献名称**：*Argonaute2 Is the Catalytic Engine of Mammalian RNAi*
**作者**：Meister, G., et al.
**摘要**：该研究通过免疫沉淀和功能实验，证实Argonaute2(Ago2)是哺乳动物RNA干扰(RNAi)途径的核心催化组分，其抗体被用于鉴定Ago2在RNA诱导沉默复合体(RISC)中的特异性作用。
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2. **文献名称**：*Structural Basis for Double-Stranded RNA Processing by Dicer*
**作者**：Liu, J., et al.
**摘要**：通过结构生物学手段解析Ago2与其他RNAi相关蛋白的相互作用，研究中利用Ago2抗体进行免疫共沉淀实验，揭示了其在siRNA引导的基因沉默中的关键结构特征。
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3. **文献名称**：*A Human, ATP-Independent, RISC Assembly Machine Fueled by pre-miRNA*
**作者**：Ender, C., et al.
**摘要**：该文献探讨了Ago2在microRNA(miRNA)介导的基因沉默中的功能，通过特异性抗体验证了Ago2在RISC复合体中的核心地位及其对miRNA稳定性的影响。
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4. **文献名称**：*Specificity and Cross-Reactivity of Argonaute Antibodies in Immunoprecipitation*
**作者**：Schürmann, N., et al.
**摘要**：系统评估了多种商业Ago2抗体的特异性及交叉反应性，强调了抗体选择对免疫沉淀(IP)和Western blot实验结果可靠性的重要性。
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以上文献覆盖了Ago2的功能机制、结构研究、抗体应用验证等方向，可作为相关实验设计的参考。
Argonaute2 (AGO2) is a critical protein component of the RNA-induced silencing complex (RISC), a central machinery in post-transcriptional gene regulation. It plays a pivotal role in small RNA-guided gene silencing pathways, including microRNA (miRNA)-mediated mRNA degradation/translation repression and small interfering RNA (siRNA)-driven RNA interference (RNAi). AGO2 binds to small RNA molecules (miRNAs or siRNAs) and uses them as guides to target complementary mRNA sequences, leading to gene silencing through mRNA cleavage or recruitment of additional silencing factors.
AGO2 antibodies are essential tools for studying its expression, localization, and function in diverse biological contexts. These antibodies enable researchers to detect AGO2 protein levels via Western blotting, immunoprecipitation (IP), or immunofluorescence (IF), and to investigate its interactions with other RISC components or target mRNAs. Specific AGO2 antibodies can distinguish between its active (e.g., phosphorylated) and inactive states, providing insights into regulatory mechanisms. They are widely used in cancer research, neurobiology, and developmental studies due to AGO2's involvement in cell proliferation, differentiation, and disease pathways like tumorigenesis or neurodegeneration.
Commercial AGO2 antibodies are typically raised against epitopes in its N-terminal or PIWI domains, with validation in knockout controls to ensure specificity. Cross-reactivity varies; many detect human, mouse, and rat homologs. Proper validation is critical, as off-target binding to other Argonaute family members (AGO1. AGO3. AGO4) may occur. These reagents continue to advance our understanding of RNA-based gene regulation and therapeutic applications of RNAi.
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