| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GPX1; GSHPX1; MGC14399; MGC88245 ; Glutathione Peroxidase 1;;GPX1 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human GPX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与GPX1抗体相关的文献及其摘要概括：
1. **文献名称**: "GPX1-mediated metabolic adaptation regulates NSCLC progression"
**作者**: Zhang Y. et al. (2022)
**摘要**: 该研究利用GPX1特异性抗体进行免疫印迹分析，发现非小细胞肺癌中GPX1蛋白表达水平与肿瘤代谢重编程相关，高表达患者生存率较低。
2. **文献名称**: "Antioxidant enzyme GPX1 as a biomarker in Alzheimer's disease"
**作者**: Smith J.R. & Lee C. (2020)
**摘要**: 通过免疫组化技术结合GPX1抗体，发现阿尔茨海默病患者脑组织中GPX1表达显著降低，提示氧化应激损伤可能加剧神经退行性病变。
3. **文献名称**: "Validation of a commercial GPX1 antibody for redox studies"
**作者**: Müller S. et al. (2019)
**摘要**: 系统验证了某品牌GPX1抗体的特异性与灵敏度，确认其在人/小鼠细胞系Western blot及免疫荧光中的可靠性，为氧化还原研究提供工具支持。
4. **文献名称**: "GPX1 deficiency promotes hepatocellular carcinoma via NRF2 pathway"
**作者**: Wang L. et al. (2021)
**摘要**: 使用GPX1敲除小鼠模型及抗体检测，揭示GPX1缺失通过激活NRF2通路促进肝癌发生，提示其作为潜在治疗靶点。
注：以上为示例性内容，实际文献需通过数据库(如PubMed)检索确认。
**Background of GPX1 Antibody**
Glutathione peroxidase 1 (GPX1) is a key antioxidant enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides, protecting cells from oxidative damage. As a member of the glutathione peroxidase family, GPX1 utilizes glutathione as a co-substrate to neutralize reactive oxygen species (ROS), playing a critical role in maintaining cellular redox homeostasis. Dysregulation of GPX1 has been implicated in various pathologies, including cancer, neurodegenerative disorders, cardiovascular diseases, and diabetes, making it a significant research target.
GPX1 antibodies are essential tools for detecting and quantifying GPX1 expression in biological samples. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and enzyme-linked immunosorbent assays (ELISA). They enable researchers to study GPX1 localization, expression levels, and interactions under different physiological or pathological conditions. Polyclonal and monoclonal GPX1 antibodies are available, each offering distinct advantages in specificity and sensitivity.
Validation of GPX1 antibodies is crucial, as cross-reactivity with other GPX isoforms (e.g., GPX2-GPX8) can occur. Knockout cell lines or tissues are often employed to confirm antibody specificity. Commercially sourced GPX1 antibodies are typically raised in hosts like rabbits or mice, with immunogens derived from human or mouse GPX1 sequences. Research involving GPX1 antibodies continues to advance understanding of oxidative stress-related mechanisms and therapeutic strategies targeting antioxidant pathways.
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