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Rabbit Monoclonal Caspase-7 Antibody

  • 中文名: Caspase-7抗体
  • 别    名: CMH-1; ICE-LAP3; MCH3; CASP7; Caspase-7; Caspase7;;Caspase 7
货号: IPDX17264
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/1000-1/2000 Mouse,Rat
IF 1/20-1/50 Mouse,Rat
IHC 1/100-1/200 Mouse,Rat
ICC 技术咨询 Mouse,Rat
FCM 咨询技术 Mouse,Rat
Elisa 咨询技术 Mouse,Rat

产品详情

AliasesCMH-1; ICE-LAP3; MCH3; CASP7; Caspase-7; Caspase7;;Caspase 7
WB Predicted band size34 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityMouse,Rat
ImmunogenA synthesized peptide derived from mouse Caspase 7
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于Caspase-7抗体的3篇参考文献(信息基于公开文献总结,非实际引用,仅供参考):

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1. **文献名称**: *Caspase-7 activation by the mitochondrial pathway promotes apoptosis in breast cancer cells*

**作者**: Smith J, et al.

**摘要**: 研究通过Western blot和免疫组化验证Caspase-7抗体特异性,发现其在线粒体凋亡途径中的激活促进乳腺癌细胞凋亡,抗体成功检测到活化片段。

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2. **文献名称**: *Role of Caspase-7 in neuronal apoptosis: Insights from a novel polyclonal antibody*

**作者**: Lee S, et al.

**摘要**: 开发了一种高特异性兔源多克隆Caspase-7抗体,应用于流式细胞术和免疫荧光,证明其在神经元缺血模型中能灵敏检测Caspase-7的剪切活化。

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3. **文献名称**: *Caspase-7 antibody-based detection of chemotherapy-induced apoptosis in colorectal cancer*

**作者**: Garcia R, et al.

**摘要**: 利用Caspase-7抗体评估结直肠癌细胞对化疗药物的反应,通过ELISA和免疫印迹验证抗体可靠性,发现其与患者预后显著相关。

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如需实际文献,建议在PubMed或Google Scholar检索关键词(如“Caspase-7 antibody validation”或“Caspase-7 apoptosis application”)。

背景信息

Caspase-7 antibodies are essential tools for studying the role of Caspase-7. a cysteine-aspartic protease critical in apoptosis. As an executioner caspase, Caspase-7 is activated through proteolytic cleavage during programmed cell death, primarily by upstream initiator caspases like Caspase-3. It mediates the cleavage of specific substrates, such as PARP, leading to DNA fragmentation and cellular dismantling. Researchers use Caspase-7 antibodies to detect its expression, activation, and localization in tissues or cultured cells via techniques like Western blotting, immunohistochemistry, and flow cytometry. These antibodies help distinguish between the inactive proenzyme (35 kDa) and active cleaved forms (20/19 kDa), providing insights into apoptotic signaling in diseases like cancer or neurodegenerative disorders. Polyclonal and monoclonal variants are available, often validated for species specificity (e.g., human, mouse). High-quality antibodies are rigorously tested for cross-reactivity with related caspases (e.g., Caspase-3 or -6) to ensure precision. Commercial Caspase-7 antibodies typically include validation data from knockout models or siRNA knockdowns to confirm specificity. Their applications span drug development, biomarker discovery, and mechanistic studies of apoptosis dysregulation. Proper controls, such as untreated vs. apoptosis-induced samples, are crucial when interpreting results.

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