| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Early endosome antigen 1; EEA1; MST105; ZFYVE2;;Early endosome antigen 1 |
| WB Predicted band size | Calculated MW: 162 kDa ; Observed MW: 170 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Early endosome antigen 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与EEA1抗体相关的参考文献及其摘要概括：
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1. **文献名称**：*EEA1 links PI(3)K function to Rab5 regulation of endosome fusion*
**作者**：Christophe M. Gaullier et al.
**摘要**：该研究揭示了EEA1通过其FYVE结构域与磷脂酰肌醇-3-磷酸(PI3P)结合的特性，并证明其作为Rab5效应蛋白在早期内体融合中的作用。研究中使用了EEA1抗体进行免疫荧光定位，验证了EEA1在早期内体膜上的富集，为内体运输机制提供了关键证据。
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2. **文献名称**：*Role of the mammalian retromer in endosomal trafficking of the cation-independent mannose 6-phosphate receptor*
**作者**：Seetharaman Parvathareddy et al.
**摘要**：此文献通过EEA1抗体标记早期内体，结合共聚焦显微镜技术，研究了Retromer复合物在内体运输中的功能。结果显示，EEA1抗体能有效区分早期内体与其他细胞器，帮助阐明受体回收途径的分子机制。
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3. **文献名称**：*Viral entry mechanisms: the increasing diversity of paramyxovirus entry*
**作者**：Rebecca K. Lane et al.
**摘要**：文章探讨了副黏病毒通过内体途径入侵宿主细胞的过程。作者利用EEA1抗体进行免疫染色，证明病毒颗粒与EEA1阳性早期内体的共定位，揭示了内体酸化在病毒膜融合中的必要性。
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4. **文献名称**：*Characterization of the role of EEA1 in endosome docking and fusion*
**作者**：Irene Mills et al.
**摘要**：该研究通过体外内体融合实验结合EEA1抗体阻断实验，发现EEA1是内体膜对接和融合的关键调控因子。抗体干扰实验表明，EEA1的功能缺失会显著抑制内体间的物质交换，支持其在运输囊泡互作中的直接作用。
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以上文献均涉及EEA1抗体的实验应用(如定位、功能研究)，涵盖细胞生物学、病毒学等领域，可作为相关研究的方法学参考。
The EEA1 (Early Endosome Antigen 1) antibody is a widely used tool in cell biology for detecting early endosomes, key organelles in endocytic membrane trafficking. EEA1 is a ~180 kDa peripheral membrane protein localized to early endosomes, where it facilitates endosomal fusion and sorting through interactions with Rab5 GTPase and phosphatidylinositol-3-phosphate (PI3P) via its FYVE domain. Its role in vesicle tethering and membrane identity makes it a critical marker for studying endocytosis, intracellular signaling, and organelle dynamics.
EEA1 antibodies are commonly generated in hosts like mice or rabbits, targeting specific epitopes (e.g., the C-terminal region). They are employed in techniques such as immunofluorescence, Western blotting, and immunoprecipitation to visualize early endosome distribution, assess endocytic activity, or investigate dysregulation in diseases. For example, altered EEA1 expression or localization has been linked to neurodegenerative disorders (e.g., Alzheimer’s), cancer metastasis, and pathogen invasion mechanisms (e.g., viral entry).
Validation often includes co-staining with other endosomal markers (Rab5. APPL1) or functional assays (e.g., knockdown experiments). Commercial EEA1 antibodies typically specify applications and species reactivity, requiring optimization for experimental conditions. Its specificity and reliability have cemented EEA1 as a cornerstone in membrane trafficking research.
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