| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3 histone; HIST1H3A; Histone cluster 1, H3a; H3K18me1;;MonoMethyl-Histone H3 (K19) |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the methylation site of K19 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Histone H3 (monomethyl K18) 抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*"Site-specific methylation of histone H3K18 promotes DNA damage response initiation"*
**作者**：Smith ER, et al.
**摘要**：该研究揭示了组蛋白H3第18位赖氨酸单甲基化(H3K18me1)在DNA损伤应答中的调控作用。通过开发特异性抗体，作者证明H3K18me1在DNA损伤位点富集，并促进ATM激酶介导的修复信号传导。
2. **文献名称**：*"Dynamic regulation of histone H3K18 methylation during cell cycle progression"*
**作者**：Li B, et al.
**摘要**：本文利用H3K18me1特异性抗体，通过免疫荧光和ChIP-seq技术，揭示了该修饰在细胞周期中的动态变化。研究发现H3K18me1在G1期显著富集于异染色质区域，可能参与维持基因组稳定性。
3. **文献名称**：*"H3K18 monomethylation marks active enhancers in embryonic stem cells"*
**作者**：Zhao Y, et al.
**摘要**：该研究通过H3K18me1抗体结合染色质分析，发现该修饰特异性地标记胚胎干细胞中的活跃增强子区域，并调控多能性相关基因的表达，提示其在细胞命运决定中的表观遗传功能。
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注：以上文献信息为示例性内容，实际文献需通过PubMed或学术数据库检索确认具体标题及作者。如需真实文献，可搜索关键词“H3K18me1 antibody”或“histone H3 monomethylation K18”获取相关研究。
The histone H3 monomethylated at lysine 18 (H3K18me1) antibody is a critical tool for studying epigenetic regulation linked to gene expression and chromatin dynamics. Histone H3. a core component of nucleosomes, undergoes post-translational modifications (PTMs) such as methylation, which influence DNA accessibility and transcriptional activity. Monomethylation at lysine 18 (K18) is a conserved mark associated with both active and repressive chromatin states, depending on cellular context. H3K18me1 has been implicated in regulating developmental genes, maintaining stem cell pluripotency, and modulating DNA damage response pathways. Dysregulation of this mark is observed in cancers, where altered H3K18me1 levels correlate with tumor progression and patient prognosis.
This antibody specifically recognizes the monomethylated form of H3K18. distinguishing it from unmethylated, dimethylated, or trimethylated states. It is commonly used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to map H3K18me1 distribution across genomes or assess its abundance in cellular models. Researchers validate its specificity using knockout controls or competitive peptides. Studies employing this antibody have shed light on how H3K18me1 interacts with chromatin modifiers (e.g., SETD6 methyltransferase) and readers to orchestrate transcriptional programs. Its application continues to advance understanding of epigenetic mechanisms in development, disease, and therapeutic targeting.
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