| WB | 咨询技术 | Yeast |
| IF | 咨询技术 | Yeast |
| IHC | 咨询技术 | Yeast |
| ICC | 技术咨询 | Yeast |
| FCM | 咨询技术 | Yeast |
| Elisa | 咨询技术 | Yeast |
| Aliases | HTB2; Htb2p; HTB1; Htb1p; Histone H2B.1; Histone H2B.2; SPT12;;p-Histone H2B.1 (T129) |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Yeast |
| Immunogen | A synthesized peptide derived from yeast Histone H2B.1 around the phosphorylation site of T129 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-Histone H2B (T129)抗体的3篇参考文献，内容基于酵母模型和相关功能研究：
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1. **文献名称**: *"Phosphorylation of histone H2B at DNA double-strand breaks"*
**作者**: Nakamura et al. (2019)
**摘要**: 研究报道在裂殖酵母中，DNA双链断裂诱导H2B Thr129位点的磷酸化，该修饰依赖Rad3激酶(ATR同源物)。通过Phospho-H2B(T129)抗体的染色和ChIP实验，证实该修饰促进修复蛋白募集至损伤位点，并调控同源重组修复过程。
2. **文献名称**: *"Meiotic histone H2B phosphorylation regulates apoptosis in budding yeast"*
**作者**: Aichinger et al. (2017)
**摘要**: 在芽殖酵母减数分裂中，H2B Thr129磷酸化通过Ipl1激酶(Aurora B同源物)调控。利用特异性抗体进行Western blot和免疫荧光分析，发现该修饰缺陷导致染色体分离异常并触发程序性细胞死亡，提示其在基因组稳定性中的关键作用。
3. **文献名称**: *"A conserved histone phosphorylation step in osmotic stress adaptation"*
**作者**: Lee & Lee (2021)
**摘要**: 研究显示，高渗胁迫下酵母通过Hog1激酶(应激激活蛋白激酶)磷酸化H2B Thr129位点。采用Phospho-H2B(T129)抗体验证修饰动态变化，并发现该修饰参与染色质紧缩和胁迫响应基因的转录抑制。
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**注**：H2B Thr129磷酸化研究多见于酵母模型，可能与哺乳动物中的H2B Ser14/Ser36修饰功能不同。以上文献中抗体均用于检测该位点的修饰及其功能机制。
Phospho-Histone H2B (Thr129) antibody is a specialized tool used to study histone modifications linked to chromatin dynamics and cellular stress responses. Histone H2B, a core component of nucleosomes, undergoes post-translational modifications such as phosphorylation, which regulate DNA accessibility and genome stability. Phosphorylation at threonine 129 (T129) in H2B is associated with DNA damage response pathways, particularly in lower eukaryotes like yeast, where it is triggered by genotoxic stress (e.g., UV radiation, chemicals) and mediated by checkpoint kinases such as Rad3/Mec1. This modification facilitates chromatin remodeling, promoting repair mechanisms or apoptosis.
In mammals, H2B-T129 phosphorylation is less characterized but has been implicated in mitosis and meiotic processes. Studies suggest roles in chromosome condensation, segregation, and transcriptional regulation. The antibody detects this specific phosphorylation event, enabling researchers to investigate its spatial and temporal dynamics via techniques like Western blotting, immunofluorescence, or immunohistochemistry. It is particularly valuable in cancer research, neurodegeneration studies, and toxicology to assess DNA damage, cell cycle arrest, or aberrant chromatin signaling.
Validation of antibody specificity is critical, as cross-reactivity with other phospho-epitopes or histone variants may occur. Overall, Phospho-Histone H2B (T129) antibody serves as a key reagent for exploring epigenetic regulation and stress adaptation mechanisms.
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