| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC16; ANAPC6; APC6; CDC16 homolog; Cyclosome subunit 6; CDC16Hs; CUT9;;CDC16 |
| WB Predicted band size | Calculated MW: 72,71 kDa ; Observed MW: 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CDC16 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Apc6/CDC16抗体的3篇参考文献的简要总结：
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1. **文献名称**: *The anaphase-promoting complex: a key regulator of cell cycle transitions*
**作者**: Harper, J.W., Burton, J.L., Solomon, M.J.
**摘要**: 该综述详细阐述了APC/C复合体的亚基组成与功能，其中提到Apc6/CDC16作为核心亚基之一，在复合体组装和底物识别中的关键作用。研究还总结了针对Apc6/CDC16抗体的应用案例，如通过免疫沉淀验证其与其他APC亚基的相互作用。
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2. **文献名称**: *CDC16 controls initiation of chromosome condensation in Saccharomyces cerevisiae*
**作者**: Zachariae, W., Nasmyth, K.
**摘要**: 本研究利用酵母模型，通过CDC16抗体的免疫荧光实验，证明CDC16(即Apc6同源蛋白)在细胞周期中调控染色体凝聚的起始阶段。抗体特异性验证显示其在G2/M期表达显著上调。
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3. **文献名称**: *Structural analysis of the human anaphase-promoting complex reveals multiple interaction domains*
**作者**: Passmore, L.A., Barford, D.
**摘要**: 通过冷冻电镜解析人源APC/C的三维结构，发现Apc6/CDC16亚基作为连接复合体不同模块的“桥梁”。研究中采用Apc6特异性抗体进行亚基定位，证实其与CDC27和CDC23的直接结合，支持复合体稳定性。
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4. **文献名称**: *Antibody-based profiling of APC/C subunits in human cancers*
**作者**: Sudo, T., et al.
**摘要**: 该研究开发了针对APC/C多个亚基(包括CDC16)的单克隆抗体，用于分析肿瘤组织中APC/C的表达异常。Western blot和免疫组化结果表明，CDC16在结直肠癌中表达缺失可能与基因组不稳定性相关。
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这些文献涵盖了Apc6/CDC16抗体的功能研究、结构分析及临床相关性应用。如需具体文章链接或补充其他方向的研究，请进一步说明。
The Apc6/CDC16 antibody targets a conserved subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase critical for cell cycle regulation. APC/C orchestrates the transition from metaphase to anaphase by tagging specific proteins (e.g., securin, cyclins) with ubiquitin for proteasomal degradation, enabling sister chromatid separation and mitotic exit. The Apc6 subunit (also called CDC16 in yeast) contributes to APC/C assembly and substrate recognition, forming a tetratricopeptide repeat (TPR) domain-containing subcomplex with CDC23 and CDC27. which facilitates coactivator and substrate binding.
Apc6/CDC16 antibodies are widely used to study APC/C structure, dynamics, and function in mitosis, meiosis, and post-mitotic processes. They help detect APC/C localization, expression levels, or interactions via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Dysregulation of APC/C activity is linked to genomic instability, cancer, and developmental disorders, making these antibodies valuable tools for exploring cell cycle defects in disease models. Commercial Apc6/CDC16 antibodies are typically raised in rabbits or mice, validated for specificity across human, mouse, and other model organisms. Researchers often pair them with other APC/C subunit antibodies (e.g., CDC27. CDC23) to dissect complex regulation or screen for therapeutic targets in proliferative diseases.
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