| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SHMT1 |
| Uniprot No | P34896 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMTMPVNGAHKDADLWSSHDKMLAQPLKDSD VEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQ RYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTA LVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGY INYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMA HISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKT GKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQ VVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEAC SIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIE LTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASFFPLPGL PDF |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SHMT1重组蛋白的3篇代表性文献概览：
1. **文献名称**：*Cloning, expression, and purification of human cytosolic serine hydroxymethyltransferase*
**作者**：Girgis S. et al.
**摘要**：研究报道了人源SHMT1基因的克隆及在大肠杆菌中的重组表达，优化了纯化步骤获得高活性酶，并验证了其催化丝氨酸与甘氨酸相互转化的功能。
2. **文献名称**：*Structural insights into the catalytic mechanism of human cytosolic serine hydroxymethyltransferase*
**作者**：Nixon P.F. et al.
**摘要**：通过X射线晶体学解析了重组SHMT1的三维结构，阐明了其辅酶结合位点及底物识别机制，揭示了酶催化循环的关键残基。
3. **文献名称**：*Functional characterization of SHMT1 variants in folate metabolism and DNA synthesis*
**作者**：Anderson D.D. et al.
**摘要**：利用重组SHMT1蛋白分析多个遗传变异体对叶酸代谢通路的影响，发现某些突变导致酶活性显著降低，可能与神经管发育缺陷相关。
*注*：以上文献为示例性概括，实际引用需查询具体数据库(如PubMed)。如需近期研究，可补充关键词“recombinant SHMT1 cancer”或“SHMT1 inhibitor”拓展文献范围。
**Background of SHMT1 Recombinant Protein**
Serine hydroxymethyltransferase 1 (SHMT1) is a pivotal enzyme in one-carbon metabolism, catalyzing the reversible conversion of serine to glycine while generating 5.10-methylenetetrahydrofolate. This reaction provides essential one-carbon units for nucleotide synthesis, methylation reactions, and redox homeostasis, making SHMT1 critical for cell proliferation, DNA repair, and epigenetic regulation. SHMT1 is primarily localized in the cytosol, distinguishing it from its mitochondrial isoform, SHMT2. though both isoforms share structural and functional similarities.
SHMT1’s role extends beyond basic metabolism; it is implicated in various physiological and pathological processes. Dysregulation of SHMT1 has been linked to cancer, as rapidly proliferating tumors depend on its activity to sustain nucleotide production. Additionally, genetic polymorphisms in SHMT1 are associated with increased risks of neural tube defects and cardiovascular diseases, highlighting its importance in development and systemic health.
Recombinant SHMT1 protein, typically produced in *E. coli* or eukaryotic expression systems, enables detailed biochemical and structural studies. Its production involves cloning the human SHMT1 gene into expression vectors, followed by purification using affinity chromatography (e.g., His-tag systems). Recombinant SHMT1 is widely used to investigate enzyme kinetics, substrate specificity, and inhibitor interactions, aiding drug discovery efforts targeting folate-dependent metabolism in cancer and other hyperproliferative disorders. Furthermore, it serves as a tool to study metabolic adaptations in cellular models and explore SHMT1’s interplay with other one-carbon pathway enzymes.
Research on SHMT1 continues to uncover its regulatory mechanisms, including post-translational modifications and allosteric regulation, offering insights into metabolic flexibility in health and disease.
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