Mouse Monoclonal PPM1A Antibody

The PPM1A antibody is a crucial tool for studying the protein phosphatase magnesium-dependent 1A (PPM1A), a member of the PPM family of serine/threonine phosphatases. PPM1A, also known as PP2Cα, plays vital roles in cellular stress responses, apoptosis, and proliferation by dephosphorylating key signaling molecules. It regulates multiple pathways, including TGF-β, p38 MAPK, and NF-κB, influencing processes like inflammation, cell cycle control, and DNA damage repair. Researchers use PPM1A antibodies in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to detect protein expression levels, subcellular localization, and interactions in various biological samples. These antibodies are typically raised in hosts like rabbits or mice, with monoclonal or polyclonal variants available. Specificity and affinity are critical parameters for experimental reliability, requiring validation via knockout controls or peptide blocking. Dysregulation of PPM1A has been implicated in cancer, neurodegenerative diseases, and metabolic disorders, making its study relevant for therapeutic development. The antibody's utility extends to both basic research and clinical investigations, aiding in uncovering PPM1A's physiological and pathological mechanisms. Proper experimental optimization, including concentration and buffer conditions, is essential to ensure accurate detection given potential cross-reactivity with related phosphatases.