| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HNRNPK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与HNRNPK抗体相关的代表性文献概览：
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1. **文献名称**：*Heterogeneous Nuclear Ribonucleoprotein K Is a Novel Regulator of p53-dependent Transcription in DNA Damage Response*
**作者**：Moumen, A. et al. (2012)
**摘要**：研究揭示了HNRNPK通过与肿瘤抑制蛋白p53的直接互作，在DNA损伤应答中调控靶基因转录的机制。实验使用HNRNPK特异性抗体进行染色质免疫共沉淀(ChIP)和Western blot验证，证实其在DNA修复通路中的关键作用。
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2. **文献名称**：*HNRNPK promotes metastatic colonization by modulating EGFR translation and extracellular vesicle secretion in breast cancer*
**作者**：Chen, Y. et al. (2020)
**摘要**：该研究通过免疫组化(IHC)和免疫荧光(IF)技术，利用HNRNPK抗体分析乳腺癌组织样本，发现其高表达与转移相关。机制研究表明，HNRNPK通过调控EGFR翻译和外泌体分泌促进肿瘤转移。
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3. **文献名称**：*Proteomic analysis identifies hnRNPK as a novel stress granule component and prognostic marker in hepatocellular carcinoma*
**作者**：Huang, S. et al. (2019)
**摘要**：通过蛋白质组学结合HNRNPK抗体进行免疫沉淀，研究发现HNRNPK在肝癌细胞应激颗粒中的富集，并与患者预后不良相关。实验验证了其作为潜在生物标志物的价值。
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(注：上述文献为示例，实际引用时建议通过PubMed或Google Scholar核对具体信息。)
The heterogeneous nuclear ribonucleoprotein K (HNRNPK) is a multifunctional RNA-binding protein involved in transcriptional and post-transcriptional regulation of gene expression. It belongs to the hnRNP family and plays critical roles in RNA processing, DNA repair, chromatin remodeling, and cellular stress responses. HNRNPK interacts with nucleic acids (RNA/DNA) and proteins through its three K-homology (KH) domains, acting as a molecular scaffold to integrate signaling pathways. Its functions span mRNA stabilization, splicing, translation, and epigenetic regulation, impacting cell proliferation, differentiation, and apoptosis.
HNRNPK antibodies are essential tools for studying its expression, localization, and interactions in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. Dysregulation of HNRNPK is linked to cancers (e.g., leukemia, breast, and liver cancer), neurological disorders, and developmental defects, making its detection crucial for understanding disease mechanisms. Research using HNRNPK antibodies has highlighted its role in oncogenic signaling, viral replication, and stress granule formation, underscoring its potential as a therapeutic or diagnostic target. Validation of antibody specificity remains critical due to structural similarities among KH domain proteins.
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