| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PLCB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PLCB1抗体的3-4篇模拟参考文献示例(实际文献需通过学术数据库核实)：
1. **文献名称**：*"PLCB1 modulates synaptic plasticity via glutamate receptor trafficking in hippocampal neurons"*
**作者**：Chen L, et al.
**摘要**：研究通过PLCB1特异性抗体进行免疫荧光和Western blot分析，发现PLCB1通过调控AMPA受体亚细胞定位影响突触可塑性，提示其在学习和记忆中的潜在作用。
2. **文献名称**：*"Aberrant expression of PLCB1 in colorectal cancer and its clinical implications"*
**作者**：Wang Y, et al.
**摘要**：利用PLCB1单克隆抗体对结直肠癌组织进行免疫组化分析，发现PLCB1高表达与患者预后不良相关，并证实其在癌细胞增殖和迁移中的功能。
3. **文献名称**：*"Development of a high-affinity monoclonal antibody against human PLCB1 for diagnostic applications"*
**作者**：Kimura T, et al.
**摘要**：报道了一种新型PLCB1单克隆抗体的开发与验证，该抗体在ELISA和免疫印染中表现出高特异性，适用于神经系统疾病的生物标志物检测。
4. **文献名称**：*"PLCB1 deficiency disrupts cortical development via impaired mTOR signaling"*
**作者**：Rodriguez S, et al.
**摘要**：通过PLCB1抗体在小鼠脑组织中的免疫定位研究，发现PLCB1缺失导致mTOR通路异常，进而影响神经元迁移和皮层发育。
(注：以上文献为示例性概括，实际引用需查阅PubMed、Web of Science等平台获取真实信息。)
The phospholipase C beta 1 (PLCB1) antibody is a tool used to detect and study the PLCB1 protein, a key enzyme in intracellular signaling pathways. PLCB1 belongs to the phospholipase C (PLC) family, which hydrolyzes phosphatidylinositol 4.5-bisphosphate (PIP2) into inositol 1.4.5-trisphosphate (IP3) and diacylglycerol (DAG), secondary messengers that regulate calcium release and protein kinase C (PKC) activation. PLCB1 is primarily activated by G-protein-coupled receptors (GPCRs) via Gαq/11 subunits or receptor tyrosine kinases, making it critical in cellular processes like proliferation, differentiation, and neurotransmitter release.
PLCB1 antibodies are widely used in research to investigate its expression, localization, and function in tissues, particularly in the brain, where PLCB1 is highly expressed and implicated in synaptic plasticity and neurological disorders. These antibodies (often raised in rabbits or mice) are validated for applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Dysregulation of PLCB1 has been linked to conditions like bipolar disorder, epilepsy, and certain cancers, driving interest in its mechanistic role. Researchers also utilize PLCB1 antibodies to explore interactions with signaling partners or mutations affecting enzyme activity. Proper antibody validation (e.g., knockout controls) is essential to ensure specificity, given the structural homology among PLC isoforms.
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